男性生殖道沙眼衣原体血清分型及其ompA基因变异分析

Serotypic distribution of Chlamydia trachomatis infection of genital tract in males

目的 了解男性生殖道沙眼衣原体感染患者沙眼衣原体血清型分布及其主要外膜蛋白编码基因ompA基因变异情况.方法 收集2011年12月至2013年3月首都医科大学附属北京同仁医院泌尿门诊疑似生殖道沙眼衣原体感染的男性患者送检的生殖道分泌物标本399份,使用沙眼衣原体检测试剂盒进行初筛,将初筛阳性标本进行巢式PCR扩增其ompA基因并测序,测序产物使用BLAST比对分型,并使用MegAlign程序进行比对.结果 399份标本中检测出沙眼衣原体的标本有59份,沙眼衣原体检出阳性率为14.8%（59/399）.59份阳性标本均成功分型,其中E型17份（28.8%）,F型16份（27.1%）,D型13份（22.0%）,G型5份（8.5%）,H、K型各3份（5.1%）,J型 2份（3.4%）;其中血清型D型临床株ompA基因突变最多（9株）,且多为有意义突变,其次为G型（5株）.结论 男性生殖道沙眼衣原体血清型以E、F、D型为主;其中血清型D型ompA基因变异最多.

Objective To explore the serotypic distribution and ompA gene variations of Chlamydia trachomatis （ C. trachomatis） of male reproductive tract at our hospital. Methods A total of 399 samples of reproductive tract secretion were collected from urinary outpatient with suspected C. trachomatis infection of genital tract from December 2011 to March 2013 and screened by C. trachomatis detection kit. Amplificated by nested PCR and sequenced of C. trachomatis ompA gene after screening positive samples. Sequencing products were typed by BLAST of PubMed and MegAlign program of I）NAstar software to compare the clinical isolates of C. trachomatis ompA gene with the standard sequence. And various serotypes of C. trachomatis ompA gene mutation were analyzed. Results C. trachomatis was detected with a positive rate of 14. 8% （59/399）. All ompA genes of 59 C. trachomatis positive specimens were successfully amplified and serotyped. The types were E （n = 17,28. 8%）, F （n = 16,27.1%）, D （n = 13,22. 0%）, G （n = 5, 8.5 % ）, H （ n = 3,5.1% ）, K （ n = 3,5.1% ） and J （ n = 2,3.4% ）. The most mutations of ompA gene of C. trachomatis in clinical specimens were serotype D （n = 9 ）, followed by serotype G （n = 5 ）. Conclusions The distribution of serotypes in male patients is serotypes E, F, D-type dominant. The serotype D of C. trachomatis is variation up.