摘要
The acute toxicity of five phenolic compounds each to 15 d old Artemia sinica was determined in this study. The brineshrimp A. sinica was hatched from the encysted dry eggs (Bohai Bay Brand) produced by Dongying Ocean Artemia Co., Ltd., China at27 °C ± 1 °C in pre-filtered (through pores of 0.45 μm in diameter) and autoclaved seawater (salinity 31, pH 7.5-8.0) in a cilindroconicalglass beaker (2000mL in volume) under continuous illumination (provided by a side set 20W fluorescent lamp) with slight aeration.Ten A rtemia individuals from the same batch of the hatched were cultured in 10 mL toxicant solution prepared with seawater (salinity31, pH 7.5-8.0) at room temperature (about 20°C) to determine 24h, 48h and 72h medium lethal concentration (LCs0) of 5 phenoliccompounds each. It was found that the toxicity of n-heptylphenol was the highest followed by nonylphenol, t-butylphenol,2,4-dichlorophenol and bisphenol A in order. The LC50 values of the 5 compounds were calculated with regression analysis. The realconcentration (in μg L-1) of 5 phenolic compounds each in toxicant solutions was measured with GC/MS analysis. Significant loss ofphenolic compounds caused by either adsorption or desorption was not found. The significant difference of LCs0 values was foundamong the five compounds 3 exposure times each. The range between the highest no-observed-effect concentration (NOEC) and 100%death causing concentration of five phenolic compounds each was determined. The toxicity in term of 24 h LC50 value of n-HP was 9.10times higher than that of BPA, 1.71 times higher than t-BP, 1.53 times higher than 2,4-DCP and 1.36 times higher than NP, respectively.
The acute toxicity of five phenolic compounds each to 15 d old Artemia sinica was determined in this study. The brineshrimp A. sinica was hatched from the encysted dry eggs (Bohai Bay Brand) produced by Dongying Ocean Artemia Co., Ltd., China at27 °C ± 1 °C in pre-filtered (through pores of 0.45 μm in diameter) and autoclaved seawater (salinity 31, pH 7.5-8.0) in a cilindroconicalglass beaker (2000mL in volume) under continuous illumination (provided by a side set 20W fluorescent lamp) with slight aeration.Ten A rtemia individuals from the same batch of the hatched were cultured in 10 mL toxicant solution prepared with seawater (salinity31, pH 7.5-8.0) at room temperature (about 20°C) to determine 24h, 48h and 72h medium lethal concentration (LCs0) of 5 phenoliccompounds each. It was found that the toxicity of n-heptylphenol was the highest followed by nonylphenol, t-butylphenol,2,4-dichlorophenol and bisphenol A in order. The LC50 values of the 5 compounds were calculated with regression analysis. The realconcentration (in μg L-1) of 5 phenolic compounds each in toxicant solutions was measured with GC/MS analysis. Significant loss ofphenolic compounds caused by either adsorption or desorption was not found. The significant difference of LCs0 values was foundamong the five compounds 3 exposure times each. The range between the highest no-observed-effect concentration (NOEC) and 100%death causing concentration of five phenolic compounds each was determined. The toxicity in term of 24 h LC50 value of n-HP was 9.10times higher than that of BPA, 1.71 times higher than t-BP, 1.53 times higher than 2,4-DCP and 1.36 times higher than NP, respectively.