黄海黄杆菌YS-9412-130产酶发酵条件的优化

Optimization of fermentation conditions for enzyme production of F. yellowsea YS 9412 130

以产低温碱性蛋白酶的黄海黄杆菌 YS- 94 12 - 130突变株 SW2 - 10 4为培养菌株 ,进行碳源、氮源、无机盐、起始 p H值、培养时间、接种量和接种龄等发酵条件的优化实验。实验结果证明 ,在以1%葡萄糖为碳源 ,以 3.5%豆饼粉为氮源 ,无机盐 :0 .4 % Na2 HPO4 、0 .0 3% KH2 P0 4 、0 .0 2 % Mg SO4 、0 .1% Na2 CO3、0 .2 % Ca Cl2 ,起始 p H值 7.0 ,接种 12 h种龄的种子 4 % ,2 0℃、2 50 r/ min的条件下在旋转摇床中培养 36h,菌株产酶活性最高。

The optimization test concerning the fermentation conditions, such as carbon sources, nitrogen sources, inorganic salts, initial pH, culture time, inoculum size, inoculating time etc., is conducted with the culture strain F. yellowsea YS 9412 130 SW 2 104,the mutant of YS 9412 130.The result of the experiment shows that the optimum alkaline protease producing activity is reached after 36h of culturing in a rotary shaker(250r/min) at 20℃, with 1% glucose as carbon source; 3 5% soy bean cake meal as nitrogen source;inorganic salts of 0 4% Na 2HPO 4, 0 03% KH 2PO 4, 0 1% Na 2CO 3, 0 02% MgSO 4, 0 2% CaCl 2; initial pH of 7; 4% inoculum cultured for 12h.