黄海黄杆菌YS-9412-130低温碱性蛋白酶多克隆抗体的制备

Preparation of polyclone antibody of marine low temperature alkaline protease from F. yellowsea YS 9412 130

利用纯化的黄海黄杆菌海洋低温碱性蛋白酶 ( Marine L ow- temperature Alkaline Protease,MLAP) ,采用背皮内多点注射和皮下组织注射免疫相结合的方法对新西兰白兔进行免疫 ,制备了多克隆抗体 ,并对多克隆抗体进行了纯化。用 EL ISA的方法对其效价检测表明 ,其效价为 12 80 0 0。作者所做的

Using purified MLTAP, New Zealand white rabbit is immunized by the methods of multi point injection in butt and injection in subcutaneous tissue and the polyclone antibody is obtained. Subsequently,the polyclone antibody is purified. Detecting by ELISA method shows that the titre is 128 000. The western blotting test demonstrates that the antigen and the antibody have high specificity.