海洋低温碱性蛋白酶的遗传毒理学研究

The genetic toxicologic study on the marine low temperature alkaline proteinase

应用 NIH纯系小鼠骨髓细胞微核试验和染色体畸变试验 ,探究海洋低温碱性蛋白酶的遗传毒性作用及对人类的潜在危害。观察并计数嗜多染红细胞 ( PCE)与正红细胞 ( NRBC)的比值、微核率 ;染色体的畸变类型和畸变率 ,检测海洋低温碱性蛋白酶的诱变作用。结果表明 ,皮下注射环磷酰胺的小鼠 ,P/ N比值 <1,微核率为 2 2 .97‰ ,染色体畸变率为 18.32 % ,与生理盐水 ( NS)对照组比较差异极显著 ( P<0 .0 1) ,而海洋低温碱性蛋白酶各组小鼠微核率均 <4‰ ,染色体畸变率为 0 .19%～0 .2 5% ,与 NS组比较无显著性差异 ( P>0 .0 5)。未见海洋低温碱性蛋白酶各组对小鼠骨髓细胞的遗传毒性。

To investigate the effects of the genetic toxicology and the latent harmfulness of marine low temperature alkaline proteinase. It was used to do the micronucleus assay in bone marrow polychromatic erythrocytes and in vivo chromosome aberration test. The effects were evaluated by the ratio of polychromatic erythrocytes (PCE) and normochromatic erythrocytes(NRBC), the rate of micronucleus, chromosome aberration and the type of chromosome aberration to determine the mutagenesis of the marine low temperature alkaline proteinase. The results show that the ratio of PCE/NRBC is less than 1 and the rate of micronucleus and chromosome aberration are 22.97‰ and 18.32% respectively for the mice substaneously injected by cyclophosphamide. The effects in the mice subcutaneously injected by cyclophosphamide were significntly higher than that in control group ( P <0 01). But there are no significant differences in the effects between the groups received orally and externally the marine low temperature alkaline proteinase and control group (P >0 05). The marine low temperature alkaline proteinase has no chemical mutagen.