大闸蟹中五氯酚含量的气相色谱测定法

Determination of pentachlorophenol in Chinese mitten crab by gas chromatography

目的建立测定大闸蟹中五氯酚的气相色谱测定方法。方法大闸蟹中的五氯酚用正己烷在酸性条件下提取后用 碳酸钾反萃取，加人乙酸酐衍生化为五氯酚苯乙酸酯,用HP-5非极性毛细管色谱柱分离后，用气相色谱法（电子捕获检测 器）测定五氯酚的含量。结果五氯酚在0~ 400 pg/L浓度范围内线性关系良好,相关系数为0.999 86。方法用于大闸 蟹分析，回收率在71. 3% -88.4%之间，测定值的相对标准偏差（RSZ））为1.08%。结论该方法操作简易，精密度髙， 收率良好。

[ Objective ] To establish a method for determination of pentachlorophenol （PCP） in Chinese mitten crab by gas chroma？tography. [Methods ] PCP in Chinese mitten crab was extracted with n-hexane in acid conditions, and the extract was back-extrac？ted with potassium carbonate solution. The PCP in solution was derivatized to form PCP phenyl acetate. And it was separated on a HP-5 capillary column and detected with gas chromatography （electronic capture detector） ？ [ Results ] There was a good linear rela？tionship in the range of 0-400 pg/L of PCP, and the correlation coefficient was 0. 999 86. The proposed method was applied to a- nalysis of Chinese mitten crab,giving recoveries of 71. 3% ~ 88.4% and RSD of 1.08% . [ Conclusion]This method is easy to op？erate ,with high precision and good recoveries.