草鱼、中华鳖淋巴细胞扫描电镜结构特征及其绵羊红细胞受体(CD_2)的检测

Observation of surface microstructure and investigation of SRBC receptor (CD_2) on lymphocyte of Ctenopharyngodon idellus and Trionyx sinensis

草鱼淋巴细胞表面扫描电镜结构特征为有孔穴且较为光滑、具短小细锥状突起 ;中华鳖淋巴细胞、胸腺细胞表面扫描电镜结构特征为凹凸不平、较为光滑 ;它们均以表面凹凸不平特征的占大多数。E花环试验的光镜和扫描电镜观察显示 :草鱼淋巴细胞E花环形成不明显 ,而中华鳖淋巴细胞、胸腺细胞的成花率分别为 2 5 %～ 34%和 36 %～ 47% ,能形成花环的淋巴细胞、胸腺细胞以表面结构特征凹凸不平 (类似哺乳动物T淋巴细胞 )的为主。草鱼、中华鳖血液淋巴细胞和中华鳖胸腺细胞与抗人CD2 单克隆抗体交叉反应的免疫组化检测显示中华鳖血液淋巴细胞和胸腺细胞的CD2 阳性率分别为 2 4%和 33%。草鱼血液淋巴细胞CD2 阳性反应不明显。

Under scanning electron microscope(SEM), the surface microstructure on lymphocytes of Ctenopharyngodon idellus is characterized with full of bumpiness and holes, or rather smooth or protruding with small cones, whereas the surface microstructure on lymphocytes and thymocytes of Trionyx sinensis is characterized with full of bumpiness and holes, or rather smooth. The lymphocytes and thymocytes filled with bumpiness and holes are evidently more than others. Under optic and scanning electron microscope,the rosette formation of lymphocyte of C. idellus with SRBC is not obvious, whereas rosette forming percents in lymphocytes and thymocytes of T. sinensis are respectively 25%-34% and 36%-47%. The lymphocytes and thymocytes which form rosettes with SRBC are predominantly ones filled with bumpiness and holes (similar to T lymphocyte of mammalian). The immunol histochemistry on the cross reactivity by using monoclonal antibody against human CD 2 shows that the CD 2 positive percent of lymphocytes in peripheral blood and thymocytes of T. sinensis are respectively 24% and 33%, but no cross reactivity is found on lymphocytes in peripheral blood of C. idellus.