苯并(a)芘对大弹涂鱼肝脏DNA的损伤

DNA damage in the liver of Boleophthalmus pectinirostris with benzo(a) pyrene exposure

在实验生态条件下 ,采用碱解旋技术检测了暴露于BaP后大弹涂鱼肝脏的DNA单链断裂程度 ,结果发现DNA单链断裂程度同时受到曝污时间和曝污浓度的影响 ,并具有一定的时间—效应和剂量—效应特征 ;污染解除后DNA单链断裂程度未明显减轻 ,表明DNA损伤的修复能力较弱。以上这些结果表明DNA单链断裂适于作为BaP暴露后DNA损伤的检测指标。

DNA single strand breaks in the liver of Boleophthalmus pectinirostris with benzo(a) pyrene exposure were detected using DNA alkaline unwinding assay under experimental ecological conditions. It turned out that DNA single strand breaks were effected by exposure time and exposure concentration and were time dependent and dose related. The repair of DNA damage was weak because no significant change was observed after the fishes were transferred to clean sea water. All of the results show that DNA single strand breaks are suitable to be the bioindicator of DNA damage with BaP exposure.