表皮生长因子受体在子宫内膜样腺癌中的表达及意义

Expression of EGFR in endometrial adenocarcinoma and its clinical significance

目的研究表皮生长因子受体(EGFR)在子宫内膜样腺癌中的表达及意义。方法采用免疫组化、原位杂交和实时荧光定量PCR方法检测正常子宫内膜组织20例、不典型增生子宫内膜组织20例、子宫内膜样腺癌组织60例的EGFR蛋白、mRNA和基因表达。结果子宫内膜样腺癌组EGFR蛋白和mRNA表达高于正常子宫内膜组,差异有统计学意义(P<0.01);不典型增生子宫内膜组EGFR蛋白和mRNA表达高于正常子宫内膜组,但差异无统计学意义(P>0.05);子宫内膜样腺癌组EGFR蛋白和mRNA表达高于不典型增生子宫内膜组,但差异无统计学意义(P>0.05)。子宫内膜样腺癌组EGFR相对表达量高于正常子宫内膜组,差异有统计学意义(P<0.05),但不典型增生子宫内膜组和正常子宫内膜组比较,差异无统计学意义(P>0.05)。结论EGFR的过度表达参与子宫内膜样腺癌的发生、发展过程。

Objective To investigate the expression of EGFR in endometrial adenocarcinoma and its clinical significance. Methods The expression of EGFR protein and mRNA were detected by immunohistochemistry, in situ hybridization and re-al- time quantitative reverse transcription- polymerase chain reaction （QRT- PCR） respectively, in 20 cases of normal endometri-um, 20 cases of atypical endometrial hyperplasia and 60 cases of endometrial adenocarcinoma. Results The expressions of EGFR protein and mRNA were higher in endometrial adenocarcinoma than those in normal endometrium（P〈0.01）. There were no significant differences in expression of EGFR protein and mRNA both between normal endometrium and atypical endometrial hy-perplasia, and between adenocarcinoma and atypical hyperplasia（P〉0.05）. Conclusion The over- expression of EGFR may be involved in the occurrence and development of endometrial adenocarcinoma.