电子束辐照金花葵幼苗的生物学效应及RAPD分析

BIOLOGICAL EFFECT AND RAPD ANALYSIS OF ELECTRON IRRADIATION ON ABELMOSCHUS MANIHOT

本实验对金花葵(Abelmoschus manihot)幼苗进行剂量为0、30和50Gy的电子束辐照,结果表明,电子束辐照对金花葵的成活率有影响,在实验范围内,随着辐射剂量的增加成活率降低;经辐射处理的幼苗在生长过程中有明显的矮化现象,且50Gy处理的植株明显矮于30Gy处理的;一定剂量的电子束辐照对金花葵的开花、结实、种子的成熟等生殖生长均有影响,当剂量为50Gy时则未能结实.同时,筛选出了8个适用于金花葵的随机引物,分别为S10、S101、S168、S198、S217、S223、S248及S249.经RAPD分析显示,30和50Gy辐照的植株与对照组的遗传距离分别为0.284 0和0.376 6,30Gy辐照处理的植株所结种子与对照组所结种子的遗传距离为0.103 4.

Abelmoschus rnanihot seedlings were irradiated by electron beam at doses of 0, 30 and 50 Gy. Survival rate of Abelmoschus rnanihot was influenced by electron beam irradiation. Growth was examined and genetic variation was studied by RAPD analysis. Plants irradiated by election beam showed a dwarf phenotype, plants irradiated at 50 Gy were lower than at 30 Gy. The reproductive growth of plants such as blossom, fructification and seed maturation were influenced, and such effect increased with doses; with irradiation at 50 Gy, plants stopped seeding all together. Eight appropriate random primers were screened： S10, S101, S168, S198, S217, S223, S248 and S249. RAPD analysis showed that genetic distance between control group and 30 Gy group, 50 Gy group was 0. 284 0 and 0. 376 6 used leaves as experimental material. Genetic distance between controls seed and 30 Gy irradiated seed was 0. 103 4.