续断壮骨胶囊中总皂苷和川续断皂苷Ⅵ含量测定

Determination of Total Saponins and Asperosaponin Ⅵ in Xuduanzhuanggu Capsules

目的：建立续断壮骨胶囊中总皂苷和川续断皂苷遇的含量测定方法。方法：采用高氯酸显色，紫外-可见分光光度法测定总皂苷含量。采用高效液相色谱法测定川续断皂苷遇含量，Agilent C18柱（250 mm 215;4.6 mm，5μm），乙腈-0.15%三氟乙酸水溶液（29∶71）为流动相，检测波长215 nm ，流量1.0 mL/min。结果：总皂苷平均含量为86.3%，平均加样回收率为99.88%， RSD =1.52%；川续断皂苷遇在64～320μg/mL 范围内呈良好线性关系（r =0.9995），平均加样回收率为100.4%， RSD =0.77%。结论：所建方法准确，能快速测定续断壮骨胶囊总皂苷和川续断皂苷遇的含量，可用于该制剂的质量控制。

Objective：To establish a method for the content determination of total saponins and asperosaponin Ⅵ in xuduanzhuanggu capsules. Methods：The content of total saponins was determined by ultraviolet visible spectrophotomet-ric method using perchloric acid as the chromogenic reagent. The content of asperosaponin Ⅵ was determined by HPLC, using Agilent C18 column （250 mm ＆#215; 4.6 mm, 5 um） with acetonitrile -0.15% trifluoroacetic acid solution （29：71） as mobile phase, the detection wavelength was at 215 nm and the flow rate was 1.0 mL/min. Results： The av-erage content of total saponins was 86.3% and the average recovery was 99.88% （RSD = 1.52%）. Asperosaponin Ⅵ showed a good linear relationship in the range of 64~320 μg/mL （r=0.9995） and the average recovery was 100.4%（RSD=0.771%）. Conclusion： The established method is accurate and applicable to the rapid determination of the con-tents of total saponins and asperosaponinⅥin xuduanzhuanggu capsules , and can be used for the quality control of the preparation.