甘薯渣酶法生产葡萄糖的技术研究

Studies on Technique of Producing Glucose from Sweet Potato Residue by Enzymes

甘薯渣是以甘薯为原料生产淀粉后的残渣,其中含淀粉50%左右。通过在甘薯渣粉中加入耐高温α-淀粉酶、糖化酶和纤维素酶进行处理,然后分别对其进行保温和灭酶处理,再对糖化醪进行固液分离,浓缩得到液糖产品。经高效液相色谱(HPLC)分析发现其成分大部分为葡萄糖。利用正交试验初步得到了酶解的最佳工艺条件:料水比6%,淀粉酶加量12 U/g干物质,液化时间为60 min,糖化酶加量300 U/g干物质,纤维素酶加量0.5 U/g干物质,糖化时间为24 h。该方法工艺简单,专一性强,所得产品品质好,收率高,可有效解决甘薯渣严重污染环境的问题,且具有良好的工业应用前景。

Sweet potato residue is the by-product of sweet potato starch production. It contains about 50% starch. In this study, the alpha-amylase, glucoamylase, and cellulase were used to hydrolyze the sweet potato residue, and then were inactivated by the thermo treatment. The sugar products were separated and concentrated. Analyzed by the high performance liquid chromatography （ HPLC）, most component of the product is glucose. The condition of enzyme hydrolysis was optimized with the orthogonaal experiment, and the results are as follows, the material content is 6 percent, the amount of amylase is 12 U/g dry matter, liquefaction time is 60 min, the amount of glucoamylase is 300 U/g dry matter, the amount of cellulase is 0.5 U/g dry matter, saccharification time is 24 h. Because of its simple craft and its product with good quality and high quantity, this technique can help to solve serious environmental pollution caused by sweet potato residue, may have good industrial applications prospects.