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脂肪因子Apelin-13对3T3-L1脂肪细胞水通道蛋白7基因表达的影响

Effects of Apelin-13 on Aquaporin 7 expression in 3T3-L1 adipocytes
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摘要 目的 探讨脂肪因子Apelin-13对3T3-L1脂肪细胞水通道蛋白7（AQP7）基因表达的影响.方法 体外培养3T3-L1脂肪细胞,以油红O染色鉴定为成熟脂肪细胞后,分为阴性对照组（无干预）,不同浓度（10^-9、10^-8、10^-7、10^-6nmol/L） Apelin-13干预组（均干预24 h）,阳性对照组（10^-5 nmol/L罗格列酮干预24 h）和10^-7 nmol/L Apelin-13干预不同时间（0、12、24、36、48 h）组.用反转录-聚合酶链反应检测各组细胞AQP7 mRNA表达水平.结果 阴性对照组、10^-9、10^-8、10^-7、10^-6 nmol/L Apelin-13和阳性对照组AQP7表达水平分别为（0.22±0.02）、（0.29±0.07）、（0.36±0.05）、（0.43±0.05）、（0.31±0.06）、（0.32±0.08）,阳性对照组与阴性对照组之间差异有统计学意义（P＜0.05）;与阴性对照组比较,10^-8、10^-7 nmol/L Apelin-13能明显刺激AQP7 mRNA表达（P ＜0.05）;10^-8、10^-7 nmol/L Apelin-13组与阳性对照组比较,10^-7 nmol/L Apelin-13能明显刺激AQP7 mRNA表达（P＜0.05）;10^-8、10^-7 nmol/L Apelin-13组间差异无统计学意义.在时间组中,10^-7 nmol/L Apelin-13的0、12、24、36、48 h各组灰度比值分别为（0.27±0.09）、（0.43±0.07）、（0.55±0.10）、（0.42±0.08）、（0.33±0.09）,12、24、36 h组AQP7 mRNA表达较0h组能明显刺激AQP7 mRNA表达（P＜0.05）,作用24 h表达最高;但12、24、36h3组之间AQP7mRNA表达差异无统计学意义.结论 Apelin-13在一定程度上能增加3T3-L1脂肪细胞AQP7 mRNA表达的水平,并分别以10^-7 nmol/L和24 h为最佳作用浓度和时间. Objective To investigate a dependent manner of adipocykine Apelin-13 on Aquaporin （AQP7） gene expression in 3T3-L1 adipocyte.Methods The fully differentiated 3T3-L1 adipocytes identified by red oil O staining were divided into groups including negative control group,Apelin-13 treated groups with concentrations of 10^-9,10^-8,10^-7,10^-6 nmol/L for24 h and with concentration of 10^-7 nmol/L for0,12,24,36 and 48 h and positive control group treated with rosiglitazone for 24 h with the concentration of 10^-5 nmol/L.The expression of AQP7 mRNA was measured according to the reverse trans cription-polymerase reaction.Results The expressions of AQP7 mRNA in the negative control group,Apelin-13 treated groups with concentration of 10^-9,10^-8,10^-7,10^-6 nmol/L and positive control group were （0.22 ± 0.02）,（0.29 ± 0.07）,（0.36 ± 0.05）,（0.43 ± 0.05）,（0.31 ± 0.06） and （0.32 ± 0.08） respectively.There were significant differences between positive control group and negative control group （P 〈 0.05）; compared with negative control group,10 8,10 7 nmol/L Apelin-13 significantly stimulated AQP7 mRNA expression （P 〈 0.05） ; 10 8,10^-7 nmol/L Apelin-13 group compared with positive control group,10 7 nmol/L Apelin-13 significantly stimulated AQP7 mRNA expression （P 〈0.05）; there showed no statistical difference between 10^-8 and 10^-7 nmol/L Apelin-13 group.In the time group,the garg-scale ratio of 0,12,24,36,48 h of 10^-7 nmol/L Apelin-13 were （0.27 ±0.09）,（0.43 ± 0.07）,（0.55 ± 0.10）,（0.42 ± 0.08）,（0.33 ± 0.09） respectively.12,24,36 h group compared with 0 h group significantly stimulated AQP7 mRNA expression （P 〈 0.05）,and the highest expression at 24 h ; but there were no significant differences between the 12,24,36 h group.Conclusion To some extent,Apelin-13 increases AQP7 mRNA expression in 3T3-L1 adipocytes with the excellence concentration of 10^-7 nmol/L treatment for 24 h.
作者 陈馥 李卫平 陈永松
机构地区 汕头大学医学院第一附属医院内分泌科
出处 《中国医药》 2014年第4期570-574,共5页 China Medicine
基金 国家自然科学基金（81300310） 广东省自然科学基金（10151503102000019）
关键词 脂肪因子Apelin 3T3-L1脂肪细胞 水通道蛋白7 肥胖 Apelin 3T3-L1 adipocyte Aquaporin 7 Obesity
分类号 R335.9 [医药卫生—人体生理学]
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参考文献15

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