摘要
目的:检测人宫颈正常组织、宫颈上皮内瘤变(Cervical intraepithelial neoplasia,CIN)以及宫颈癌组织中survivin蛋白的表达定位,研究该因子与宫颈癌发生发展的关系和临床应用价值。方法:收集2年间某医院97例患者的石蜡组织标本,其中正常宫颈组织20例、CIN25例、宫颈癌52例。采用免疫组化方法检测组织切片中的survivin蛋白,研究该因子在组织中的蛋白表达定位。结果:在宫颈组织中,survivin蛋白在胞核以及胞浆内均可见阳性染色,主要定位于细胞核。正常组survivin阳性率为5.0%,CIN组阳性率为56.0%,宫颈癌组阳性率为82.7%。survivin在宫颈癌组织中的高表达,与宫颈癌的临床分期、病理分化以及淋巴结转移均呈正相关。结论:survivin与宫颈癌的发生发展密切相关,可能成为对人宫颈癌的预防、诊治,以及判断预后有价值的临床肿瘤相关指标。
Objective：To observe the expression and location of survivin protein in human normal cervices,cervical intraepithelial neoplasia and cervical carcinoma,the relationship of survivin with the occurrence,development and prognostic of cervical carcinoma,and the clinical value of survivin for cervical carcinoma.Method：All of the paraffin tissue samples were gathered from one hospital in two years.The samples were divided three groups,20 cases of normal cervices,25 cases of cervical intraepithelial neoplasia,and 52 cases of cervical carcinoma.The stains of survivin protein in the tissue samples with the immunohistochemistry methods were checked.Result：The stains of survivin in cervical tissue samples were located in cell nucleus and cytoplasm,mainly in cell nucleus.The positive rate of survivin was 5.0% in normal cervices,56.0% in cervical intraepithelial neoplasia,82.7% in cervical carcinoma.The correlation could be found of survivin with the clinical stage,histological differentiation and lymphatic metastasis of the cervical carcinoma.Conclusion：The correlation can be find of survivin with the occurance and progression the cervical carcinoma closely.Survivin will be a clinic valuable marker for the prevention,diagnosis,treatment,and judging the prognosis of human cervical carcinoma.
出处
《中国医学创新》
CAS
2014年第6期36-39,共4页
Medical Innovation of China
