野艾蒿精油对双斑萤叶甲成虫的熏蒸活性及体内酶活力的影响

Effects of Essential Oil from Artemisia lavandulaefolia DC. on Fumigation Activity and Several Enzymes Activities of Monolepta hieroglyphica(Motschulsky)Adults

测定了野艾蒿精油对双斑萤叶甲成虫的熏蒸活性及体内几种酶活力的影响。结果表明:野艾蒿精油对双斑萤叶甲成虫具有明显的熏蒸作用,野艾蒿精油剂量为10μL/L,处理8 h后校正死亡率为100%,在12 h后各精油处理组的校正死亡率均>80%。经熏蒸处理24 h后,野艾蒿精油对双斑萤叶甲成虫体内的乙酰胆碱酯酶(AChE)活力抑制作用明显;对双斑萤叶甲成虫体内的α-乙酸萘酯酶(α-NACarE)活力、酸性磷酸酯酶(ACP)活力、碱性磷酸酯酶(ALP)活力均表现出抑制作用。各处理组的谷胱甘肽-S-转移酶(GSTs)活力均高于对照组,野艾蒿精油对双斑萤叶甲成虫体内的谷胱甘肽-S-转移酶(GSTs)活力表现出明显的诱导作用。野艾蒿精油对双斑萤叶甲成虫体内过氧化物酶(POD)活力的影响不明显。

We test the effects of essential oil from Artemigate lavandulaefolia DC .on fumigation activity and several enzymes of Monolepta hieroglyphica （Motschulsky）adults .The result showed that the essen-tial oil from Artemigate lavandulaefolia DC .had obvious fumigation against Monolepta hieroglyphica （Motschulsky）adults,and the corrected mortality was 1 00%when the dose of essential oil from Artemi-gate lavandulaefolia DC .was 1 0μL/L and 8 h after treatment,and the corrected mortality of all the treatment groups reached 80%or more after 1 2 h .Essential oil from Artemigate lavandulaefolia DC .sig-nificantly inhibited AChE activity;the activities ofα-NACarE and ACP and ALP demonstrated inhibition in the body of Monolepta hieroglyphica （Motschulsky）adults .GSTs activity of all treatment groups was higher than that of the control group,and GSTs activity showed obvious induction .POD activity was not obviously affected .