BAALC蛋白在急性白血病中的表达及其临床意义

Expression significance of BAALC in acute myeloid leukemia

目的探讨脑和急性白血病胞质(Brain and acute leukemia cytoplasmic,BAALC)蛋白在急性髓细胞性白血病(Acute myeloid leukemia,AML)的表达及其临床意义。方法采用Western blot方法半定量检测BAALC蛋白在58例AML患者(初诊24例、完全缓解28例和复发6例)的骨髓单个核细胞(Bone marrow mononuclear cells,BM-MNCs)的表达水平,15例非恶性血液病患者以及AML细胞系KG1α和HL-60分别作为阴性和阳性对照。结果 KG1α和HL-60细胞BAALC均为阳性高表达,非恶性血液病对照者BAALC表达均为阴性。BAALC阳性率在AML初诊组(83.3%)与复发组(100%)均高于完全缓解(CR)组(7.1%)和阴性对照组(0)(P<0.001);AML初诊组和复发组的BAALC蛋白相对含量明显高于CR组和阴性对照组(P<0.01),且CR组和阴性对照组之间BAALC表达阳性率和相对含量差异无统计学意义(P>0.05)。结论 BAALC蛋白在AML中表达水平异常增高,提示BAALC可能与AML的发生发展密切相关。

Objective To investigate the expression and its significance of brain and acute leukemia cytoplasmic （BAALC） in acute myeilod leukemia （AML）. Methods BAALC level was assessed by Western blot in the bone marrow mononuclear cells （BM-MNCs） of 58 AML patients, including 24 newly diagnosed patients, 28 complete remission patients and 6 relapsing patients, 15 nonmalignant hematopathy patients as negative controls and AML cell line KG1 a and HL-60 as positive controls. Results The expression of BAALC was positive in KG1 a and HL-60 ceils but negative in 15 negative controls. The BAALC positive rate in AML newly diagnosed group （83.3%） and relapsing group （100%） was higher than that in complete remission （CR） group （7.1%） and negative control group （P〈0.001）. BAALC relative amount in AML newly diagnosed group and relapse group was significantly higher than that in CR group and negative control group （P〈0.01）. The positive rate and relative amount of BAALC showed no statistically difference between CR group and negative controls. Conclusion BAALC is overexpressed in AML patients, suggesting that BAALC is associated with the development of AML.