推拿影响坐骨神经损伤大鼠行为学及神经营养素3、酪氨酸激酶受体C表达的研究

Investigation of Tuina therapy on NT-3,TrkC and behavioristics of SNI model rats

目的观察推拿对坐骨神经损伤大鼠行为学、神经营养素3(neurotrophin-3,NT-3)、酪氨酸激酶受体C(tyrosine receptor kinase C,TrkC)表达的影响,研究推拿修复坐骨神经损伤的机理。方法将大鼠分为正常组、假手术组、模型组、模型对照组、推拿组,采用大鼠坐骨神经夹持损伤法于右侧股骨中段下方夹持神经进行造模,7天后进行推拿干预,共20次。干预10次、20次后,分别通过坐骨神经功能指数(sciatic functional index,SFI)、斜板试验观察各组大鼠的行为学变化;再通过免疫组化法检测各组大鼠脊髓腹角NT-3、TrkC的表达情况,最后对各组数据进行统计分析。结果模型组和模型对照组大鼠行为学检测提示坐骨神经损伤大鼠的运动功能明显降低,在推拿干预后,斜板试验明显升高,神经功能恢复情况优于模型组和模型对照组(P<0.05);SFI有一定程度恢复,负值较模型组、模型对照组高,但差异无统计学意义(P>0.05)。模型组、模型对照组及推拿组脊髓NT-3、TrkC表达与正常组相比差异均有统计学意义(P<0.05),推拿组NT-3表达与模型组相比差异也有统计学意义(P<0.05),推拿组更高。结论中医推拿可以改善神经损伤大鼠的行为学表现,可能是通过促进NT-3及其受体TrkC的表达,发挥抑制细胞凋亡,促神经元存活的作用,最终实现促进神经功能恢复,改善其运动功能。

Objective To observe the influence of Tuina therapy on nenrotrophin-3 （NT-3）, tyro- sine receptor kinase （TrkC） and behavioristics of sciatic nerve injury model rats in order to probe the mechanisms of Tuina therapy in sciatic nerve injury. Methods We divide rats into five groups which in- clude normal group, sham-operation group, model group, bondage group and Tuina group. Use the nerve clamping method to make the sciatic nerve injury model. Sciatic functional index （SFI） and oblique board test in each group are observed as behavioral evidences. Then observe the density of NT-3 and TrkC in spi- nal cord by using immunohistochemistry. Results The SFI scores of Tuina group, model group and bond- age group are significantly lower than blank control group, while Tuina group score is higher than model group, but still lower than blank control group. The oblique board test scores of the model group and bond- age group are significantly lower than blank control group, while score of Tuina group is much higher. The immunohistochemistry shows that the amount of the NT-3 and TrkC are all significantly different in model control group, bondage group and Tuina group. NT-3 and TrkC is significantly higher in Tuina group.Conclusion Tuina therapy could treats the sciatic nerve injury by increasing NT-3 and TrkC. As a result, the survival of nerve cell is promoted by Tuina therapy, then the motor function of sciatic nerve injury rats are improved.