清肠温中方对三硝基苯磺酸诱导的实验性结肠炎大鼠肠黏膜作用的研究

Effect of Qingchang Wenzhong Decoction on colonic mucosa of TNBS-induced colitis rats

目的研究清肠温中方对三硝基苯磺酸诱导的实验性结肠炎大鼠结肠黏膜的作用。方法以三硝基苯磺酸灌肠诱导大鼠急性结肠炎,造模成功后连续以清肠温中方高、中、低剂量混悬液及柳氮磺吡啶(salazosulfapyridine,SASP)混悬液灌胃治疗10天,取结肠,进行疾病活动指数(disease activity index,DAI),结肠黏膜损伤指数(colonic mucosa damage index,CMDI)和结肠组织病理学(histological score,HS)评价。结果药物干预10天后,模型组DAI、CMDI、HS明显高于正常组(P<0.05);SASP组DAI及CMDI均较模型组有明显的下降(P<0.05);清肠温中方各剂量组DAI、CMDI、HS水平较模型组均明显下降(P<0.05),其中清肠温中方高、中剂量组炎细胞侵润的程度明显低于模型组(P<0.05)。结论清肠温中方对三硝基苯磺酸诱导的结肠炎大鼠肠黏膜有保护作用,且能有效改善结肠组织炎细胞侵润。

Objective To explore the effect of Qingchang Wenzhong Decoction （QCWZD）on co- Ionic mucosa on a rat model of experimental inflammatory bowel disease （IBD）. Methods Colitis was in- duced in male sprague-dawley （SD） rats by intracolonic administration of 2,4,6-trinitrobenzene sulfonic acid （TNBS）, 100 mg/kg. QCWZD preparation and salazosulfapyridine （SASP） was given by garage route daily for 10 days. Disease activity index（DAI）, colonic mucosa damage index（ CMDI）, histological score（HS） were estimated. Results DAI, CMDI, HS was significantly higher in TNBS-treated rats （P 〈 0. 05 ） ; DAI and CMDI decreased in SASP-treated TNBS-induced colitis rats （P 〈 0. 05 ）. QCWZD prepa- ration reduced DAI, CMDI and HS of TNBS-induced rats （P 〈 0. 05 ） ; QCWZD preparation（ 1200 mg/kg, 600 mg/kg）reduced inflammatory infiltration（II） of colon（P〈0.05）. Conclusions Data obtained indi- cated that QCWZD could protect colonic mucosa by reducing inflammatory infiltration.