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标本保存温度和保存时间对流式自然杀伤细胞和B细胞数量检测的影响 被引量：2

Effect of sample storage temperature and time on NK cells and B-lymphocytes detected by flow cytometry
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摘要 目的检测标本保存时间和保存温度,为流式自然杀伤(natural killer,NK)细胞和B细胞检测标准化操作提供科学依据。方法采用四色流式细胞术分析健康体检人群外周血NK细胞和B细胞数量百分比,检测保存时间与保存温度对实验结果的影响。结果未经处理的全血标本常温(18℃)保存后检测结果稳定,低温、高温及长时间保存都引起检测结果降低;全血标本经荧光抗体染色后,用多聚甲醛固定可在4℃条件下保存1周。结论流式细胞分析法检测NK细胞和B细胞,若标本不能及时处理宜18℃保存并在96 h内检测;处理后的样本可在4℃条件下保存1周。 Objective To study the effect of storage temperature and time on the detection of NK cells and B-lymphocytes. Methods NK cells and B-lymphocytes in peripheral blood were detected by 6-color flow cytometry. The effect of storage temperature and time on the detection of NK cells and B-lymphocytes was observed. Results No change was found in the number of NK cells and B-lymphocytes in whole blood samples stored at 18℃. The number of NK cells and B-lymphocytes decreased after stored at low or high temperature. The whole blood samples that were fixed with paraformaldehyde and stained with fluorescent antibody could be stored for 7 days. Conclusion Flow cytometry can detect NK cells and B-lymphocytes. The samples can be stored at 18℃for 96 h when it is not able to treat them in time. The treated samples can be stored at 4℃for 7 days.
作者 周方圆 张蕴莉
机构地区 辽宁医学院附属第一医院检验科
出处 《解放军医学院学报》 CAS 2014年第3期283-285,共3页 Academic Journal of Chinese PLA Medical School
关键词 自然杀伤细胞 B淋巴细胞 流式细胞术 natural killer cell B-lymphocyte flow cytometry
分类号 R446 [医药卫生—诊断学]
  • 相关文献

参考文献10

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共引文献26

同被引文献19

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解放军医学院学报
2014年 第3期

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