大黄对肺卫失宣大鼠肺组织钠离子通道蛋白mRNA表达的影响

Effect of Radix et Rhizoma Rhei on Pulmonary Epithelial Sodium Channel mRNA Expression in Rats with Lung and Weifen Failing in Dispersion

【目的】观察肺卫失宣大鼠肺组织钠离子通道蛋白(ENaC)mRNA表达及大黄的调节作用。【方法】采用内毒素法建立肺卫失宣大鼠模型。选用SD大鼠随机分成正常对照组、肺卫失宣模型组、模型自然恢复5 d组、大黄预防组和大黄治疗组(剂量均为9 g·kg-1·d-1)。采用Real-time PCR法检测肺组织ENaC mRNA表达。【结果】与正常对照组比较,模型组ENaC-α﹑ENaC-β﹑ENaC-γmRNA表达均显著下降,自然恢复5 d组ENaC-α﹑ENaC-β﹑ENaC-γmRNA表达均显著上调(P<0.05);与模型组比较,大黄预防组和大黄治疗组ENaC-α﹑ENaC-β﹑ENaC-γmRNA表达明显上调;与自然恢复5 d组比较,大黄预防组和大黄治疗组ENaC-α﹑ENaC-β﹑ENaC-γmRNA表达呈明显下调作用。【结论】肺卫失宣大鼠ENaC-α﹑ENaC-β﹑ENaC-γmRNA呈双向表达异常;大黄对肺卫失宣大鼠肺组织ENaC-α﹑ENaC-β﹑ENaC-γmRNA低表达或过表达均有一定的抑制作用。

Objective To investigate the expression of epithelial sodium channel （ENaC） mRNA in the lung tissue of rats with lung and weifen failing in dispersion and to observe the regulatory effect of Radix et Rhizoma Rhei （RRR） . Methods Rat model with lung and weifen failing in dispersion was induced by intravenous injection of lipopolysaccharide （LPS） . The experimental SD rats were randomly divided into normal control group, model group, 5-day non-medication group, RRR prevention group and RRR treatment group （bothin the dose of 9g＇kg-1 ·d-1） .Real-time polymerase chain reaction （PCR） was used to observe the expression of ENaC mRNA in the lung tissue. Results Compared with the normal control group, the expression levels of ENaC alpha, beta and gamma mRNA in the model group were decreased, while those in 5-day non-medication group were elevated （P〈0.05） . The expression levels of ENaC alpha, beta and gamma mRNA in RRR prevention and treatment groups were significantly higher than those of the model group, while were lower than those of 5-day non-medication group. Conclusion The abnormal lower or higher expression of ENaC alpha, beta and gamma mRNA is shown in rat model of dispersion failure of lung and weifen, and RRR has certain inhibitory effect on the disordered ENaC mRNA expression.