土人参快速繁殖培养基的优化

Optimization of Culture Medium for Rapid Propagation of Talinum paniculatum (Jacq.) Gaertn.

【目的】采用植物组织培养技术开展土人参快速繁殖和植株再生研究，为建立土人参的快速繁殖再生体系奠定基础。【方法】以土人参的种子、嫩茎和叶片为外植体，采用正交设计法考察不同激素水平对土人参诱导、增殖、生根的影响，获得土人参最佳的诱导、增殖和生根培养基，并进一步将试管苗移栽到野外环境。【结果】以土人参种子作为外植体，选择用MS＋3．0mg／L6-苄基腺嘌呤（6-BA）培养基培养1～2周后能诱导出优质的丛生芽，再选用MS＋3．0mg／L6-BA＋1．0mg／Ld-萘乙酸（NAA）＋1．5mg／L多效唑（PP333）培养基对其进行增殖培养，可得到鲜质量增长率最大的健壮丛生芽；生根培养基MS＋0．5mg／LNAA＋0．5mg／L吲哚乙酸（IAA）＋1．0mg／L吲哚丁酸（IBA）能促进丛生芽长出直径大、长度较长、须根及绒毛较多的营养根，得到试管苗；将试管苗进行炼苗与移栽，成活率100％，且与播种栽培比较可显著缩短生长时间。[结论]建立了土人参快速繁殖再生体系．

Objective To study the rapid propagation and regeneration of Talinum p oniculatum （Jacq.） Gaertn. by plant tissue-cuhure technology, so as to lay a foundation for establishing the system of rapid propagation and regeneration. Methods Using the seeds, stems and leaves of Talinum paniculatum as the explants, we observed the effect of hormone levels on the induction, proliferation and rhizogenesis of Talinumpaniculatum by orthogonal design method, optimized the culture medium for the induction, proliferation and rhizogenesis, and transplanted the test- tube plantlet to field environment. Results High--quality sprouts were regenerated when the explants of Talinum paniculatum seeds were cultured in the culture medium composed of MS＋3.0 mg/L 6-benzyladenine （6-BA） for 1-2 week （s）, and then a cluster of strong seedlings with the highest fresh weight increase rate was regenerated after the sprouts were cultured in culture medium composed of MS ＋ 3.0 mg/L 6-BA ＋ 1.0 mg/L NAA ＋ 1.5 mg/L PP333. The rooting culture medium composed of MS ＋ 0.5 mg/L NAA ＋ 0.5 mg/L IAA＋I.0 mg/L IBA could help the sprouts regenerate large--diameter and long-length roots with more fibers and tomentums, which were easy to develop into test-tube plantlets. After the test-tube plantlets were hardened and transplanted, their survival rate arrived to 100%, and their growth time was shortened in comparison with that of sowing culture. Conclusion The rapid propagation and plant regeneration system of Talinum p aniculatum has been established.