几种基于pCAMBIA系列多用途新型植物表达载体的改建及优化

Reconstruction and Optimization of pCAMBIA-Based Plant Expression Vectors for Multifunctional Application

以pCHF3,pCAMBIA1301,pCAMBIA3300和pCAMBIA3301载体为骨架,构建CaMV 35S和rd29A启动子驱动多克隆位点(MCS:SacⅠ,KpnⅠ,SmaⅠ,BamHⅠ,XbaⅠ,SalⅠ,PstⅠ)的通用型重组植物双元表达载体,得到两种启动子驱动下MCS与eGFP融合的应用型亚细胞定位双元表达载体,并建立了对大量候选基因进行高通量筛选和功能验证的通用型表达载体系统.

To supply the required large amounts of functional recombinant plant expression vectors,a series of general plant binary expression vectors with the CaMV35S and the rd 29A promoter mediating multiple cloning sites （MCS：SacⅠ,KpnⅠ,SmaⅠ,BamⅠ,XbaⅠ,SalⅠ,P stⅠ）were constructed successfully, on the basis of pCHF3, pCAMBIA1301, pCAMBIA3300 and pCAMBIA3301 vectors,and a set of applicative binary expression vectors utilized for subcellular localization by the fusion of MCS and eGFP with these two promoters were also constructed. Subsequently, the universal high-throughput and expense-saving gene expression system was established for the scan and functional characterization of a large number of candidate genes.