三种品系小鼠对伴刀豆球蛋白A所致急性肝损伤的耐受性比较研究

Tolerance of concanavalin A in three different strains of mice with acute hepatic injury

目的应用不同剂量伴刀豆球蛋白A(ConA)诱导三种不同品系小鼠急性肝损伤,比较肝酶变化、死亡率和肝组织病理学变化。方法分别用6、12、20和30 mg·kg-1 ConA处理C57BL/6J、BABL/c和KM小鼠,8 h后对比观察急性肝损伤动物血清酶学及肝组织病理学变化情况。结果在6 mg·kg-1 ConA的作用下,三个品系小鼠均被成功制造出急性肝损伤模型;在12 mg·kg-1ConA作用下,KM小鼠血清ALT和AST分别为(1006.8±12.1)U/L和(1391.8±13.4)U/L,显著高于BABL/c小鼠[(75.7±0.5)U/L和(284.7±23.4)U/L)和C57BL/6J小鼠(104.4±32.2)U/L和(492.2±12.3)U/L,P均<0.05];同样,KM小鼠肝指数和脾指数分别为(5.4±0.3)和(0.7±0.5),均显著高于BABL/c小鼠[(5.2±0.4)和(0.6±0.3)和C57BL/6J小鼠(5.0±0.6)和(0.6±0.2),P均<0.05];在20 mg·kg-1ConA作用下,三组小鼠血清AST和ALT水平无统计学差异,但BABL/c小鼠(4/10)和C57BL/6J小鼠(5/10)死亡率显著高于KM小鼠(1/10);在30 mg·kg-1ConA作用下,三个品系小鼠死亡率均较高(KM小鼠为30%,BABL/c和C57BL/6J小鼠均为100%)。结论不同品系的小鼠对ConA的耐受性不同,KM小鼠对ConA的耐受性明显优于BABL/c小鼠和C57BL/6J小鼠,且呈剂量依赖性。

Objective To compare the differences in serum liver enzymes, mortality and liver histological changes in three different strains of mice with concanavalin A （ConA） -induced acute hepatic injury （AHI）. Methods C57BL/6J mice,BABL/c mice and KM mice were subjected to ConA at four different dosages （i.e.6, 12,20 and 30 mg·kg-1） for 8 hours,and then mice were sacrificed and serum liver enzymes and liver histological changes were examined. Results ConA at dose of 6 mg·kg-1 successfully induced acute liver injury in all three strains of mice;ConA at dose of 12 mg·kg-1 caused remarkable increases in serum ALT （1006.8 ± 12.1） U/L and AST （1391.8 ± 13.4）U/L levels in KM mice,significantly higher than those in BABL/c mice [（75.7 ± 0.5） U/L and（284.7 ± 23.4） U/L,respectively and in C57BL/6J mice （104. ±32.2） U/L and （492.2 ± 12.3） U/L,re-spectively,P〈0.05];Similarly,liver index（5.4±0.3） and spleen index（0.7±0.5） in KM mice were significantly high-er than that in BABL/c mice [（5.2±0.4） and （0.6±0.3）,respectively and in C57BL/6J mice（5.0±0.6） and （0.6±0. 2）,respectively,P〈0.05];Serum ALT and AST levels did not differ among three strains of mice when Con A at dose of 20 mg·kg -1 was used,however,the mortality in BABL/c （4/10） and C57BL/6J （5/10） was significantly higher than that in KM mice （1/10）;30 mg·kg -1 of ConA resulted in a remarkably high mortality in all three strains of mice （30% in KM mice,100% in BABL/c and 100% in C57BL/6J mice）. Conclusion Mouse strains play a significant role in liver response to Con A,and the tolerance of KM mice to ConA is better than BABL/c and C57BL/6J mice and the liver damage is in a dose-dependent manner.