利用流式细胞术检测T淋巴细胞表面分子CD3、CD4和CD8封闭效率在反复自然流产中的应用

Application of blocking efficiency detection of T lymphocytes surface molecule CD3,CD4 and CD8 by flow cytometry in recurrent spontaneous abortion

目的快速、准确检测反复自然流产(RSA)患者是否缺乏保护性封闭抗体,监测免疫治疗效果,以及探讨RSA的免疫学发生机制。方法利用流式细胞术检测T淋巴细胞表面标志CD3、CD4和CD8分子的封闭效率。结果反复自然流产患者CD3、CD4和CD8分子的封闭效率均低于健康对照组(P<0.01);利用丈夫单个核细胞进行免疫治疗后,CD3、CD4和CD8分子的封闭效率转阳率与怀孕率呈正相关性。结论利用流式细胞术检测T淋巴细胞表面CD3、CD4和CD8分子的封闭效率可以为RSA患者提供快速、准确的临床诊断依据,实现对治疗效果的监测,并有助于部分解释RSA的免疫学发病机制。

Objective To rapidly and accurately detect whether lack the protective blocking antibodies in the recurrent spontane-ous abortion （RSA） patients, to monitor the effect of immunotherapy and to study the immunological occurrence mechanism of RSA. Methods The blocking efficiency of surface markers CD3,CD4 and CD8 on T lymphocytes surface was detected by the flow cytometry. Results The blocking efficiency of CD3,CD4 and CD8 molecules in the RSA patients was lower than that in the control group （P〈0.01） ;the positive conversion rate of the blocking efficiency of CD3, CD4 and CD8 after the immunotherapy by the hus-bandrs mononuclear cell was positively correlated with the pregnant rate. Conclusion Adopting the flow cytometry to detect the blocking antibody of CD3, CD4 and CD8 on T lymphocytes surface can provide the rapid and accurate clinical diagnosis basis, realize the monitoring of the therapeutical effect and conduce to partly explain the immunological pathogenesis mechanism of RSA.