某血细胞分析仪尿红细胞MCV及RDW检出限探讨

Discussion on detection limits of urine erythrocyte MCV and RDW for a hematology analyzer

目的探讨Sysmex XS-800i血细胞分析仪可检出尿红细胞MCV及RDW的最低尿红细胞计数值。方法经人工镜检确认之血尿样本30例,所有样本不加稀释液直接在Sysmex XS-800i血细胞分析仪上以末梢血模式进行检测,取得尿红细胞参数红细胞计数(RBC)、红细胞平均体积(MCV)、红细胞体积分布宽度(RDW)初始结果。根据尿红细胞计数初始结果,对血尿样本分别进行梯度稀释或梯度离心浓缩后再经Sysmex XS-800i血细胞分析仪以末梢血模式检测尿红细胞计数、MCV、RDW,测得每个样本各参数按红细胞计数由低到高排列的系列结果,以获得该仪器对各样本可检出尿红细胞MCV及RDW的最低尿红细胞计数值。结果 Sysmex XS-800i血细胞分析仪对本研究中30例血尿样本尿红细胞计数的最低检出值为0.01×1012/L,符合厂家提供的该仪器对血红细胞计数最低检出限;Sysmex XS-800i血细胞分析仪对尿红细胞MCV、RDW的"检出限"以该仪器可检出尿红细胞MCV、RDW时最低尿红细胞计数检测值表示,本研究中不同尿液样本其值存在差异,其中可检出尿红细胞MCV最低尿红细胞计数值为0.02×1012/L^0.10×1012/L,可检出尿红细胞RDW最低尿红细胞计数值为0.04×1012/L^0.19×1012/L。结论明确血细胞分析仪对尿红细胞参数的实际"检出限"是保证尿红细胞MCV及RDW结果准确的前提条件。

Objective To discuss the lowest values of urine red blood celI（RBC） count by adopting the Sysmex XS-800i hematol-ogy analyzer to detect the mean corpuscular volume （MCV） and red blood cell volume distribution width （RDW） in urine erythro-cytes. Methods 30 hematuria samples confirmed by manual microscopic examination without dilution were detected by the Sysmex XS-800i hematology analyzer with the peripheral blood mode and the initial results were obtained including red blood cell count （RBC）, MCV and RDW. According to the initial results of urine RBC count, the hematuria samples were performed the gradient di-lution or gradient concentration by centrifuging. All the pretreated hematuria samples were detected the parameters again by the Sysmex XS-800i hematology analyzer in the peripheral blood mode,and then the series results of each sample according to the rank of RBC count from low to high were measured. Then the lowest values of urine RBC count were obtained by detecting urine eryth- rocyte MCV and RDW. Results For the 30 hematuria samples,the lowest measured value of urine RBC count detected by the Sys-mex XS-800i hematology analyzer was 0.01 × 10^12/L, which was accord with the lowest detection limit provided by the producer. The analyzer＇s lowest detection limits of urine erythrocyte MCV and RDW were expressed as the lowest value of urine RBC count. Different urine samples had difference in the lowest values of RBC count, the lowest value of urine RBC count for the detected urine RBC MCV was 0.02 × 10^12/L-0.10 × 10^12/L and which for the detected RDW was 0.04 × 10^12/L-0. 19 × 10^12/L. Conclusion Determining the real detection limits of urine RBC parameters by hematology analyzer is the premise for ensuring the accurate de- tection results of urine erythrocyte MCV and RDW.