慢性丙型肝炎患者自身抗体检测的相关因素及临床价值分析

Analysis of Related Factors and Clinical Significance for Detection Autoantibodies in Patients with Chronic Hepatitis C

目的:探讨慢性丙型肝炎患者自身抗体的阳性率及临床意义。方法:应用ELISA法检测105例慢性丙型肝炎患者血清中的抗核抗体,用免疫印迹法检测抗肝肾微粒体抗体,选择96例慢性乙型肝炎患者和80例健康人分别作为对照组。结果:105例慢性丙型肝炎患者自身抗体总阳性率为48.6%,显著高于慢性乙肝组(7.3%)和健康对照组(2.5%)。自身抗体阳性组患者的平均年龄(47.3±15.5)大于自身抗体阴性组(38.1±14.9)(P<0.05)。自身抗体阳性组中肝纤维化发生率(68.6%)高于自身抗体阴性组(48.1%)(P<0.05)。但两组间性别构成和HCV RNA阳性率无显著性差异(P>0.05)。结论:血清自身抗体的检测有助于慢性丙型肝炎的诊治和病情评估。

Objective：To investigate the positive rate and clinical significance of autoantibodies in patients with chronic hepatitis C. Methods：Anti-nuclear antibodies were detected by ELISA for 105 patients. Anti-LKM-1 were detected by Western blotting. 96 cases of patients with chronic hepatitis B and 80 healthy subjects were respectively selected for control group. Results：In chronic hepatitis C group, the total positive rate of autoantibodies was 48.6%（51/105）, which was higher than chronic hepatitis B（7.3%） or healthy group（2.5%） （P〈0.05）. In autoantibody positive group, the age was higer than that in autoantibody negative group[（47.3±15.5） vs （38.1±14.9）,P〈0.05] as well as the cirrhosis prevalence rate（62.7%vs 48.1%,P〈0.05）. There was no significance difference in both gender composition and HCV RNA positive rate in the above groups（P〉0.05）. Conclusion：Detection of serum autoantibodies are useful for diagnosis, therapy and severity evaluation in chronic hepatitis C.