赖氨酰氧化酶样-4与丝氨酸蛋白酶基因在人宫颈癌细胞中的表达

The Expression of Lysyloxidase-like 4 and Serine Protease Genes in Human Cervical Carcinoma Cell Lines in Human Cancer Cells

目的探讨赖氨酰氧化酶样-4(LOXL4)和丝氨酸蛋白酶(Matriptase)在三株人宫颈癌细胞中的表达情况。方法采用qRT-PCR及Western blot方法检测三株体外培养的宫颈癌细胞株Hela、ME180、HCC94中赖氨酰氧化酶样-4和Matriptase mRNA的表达及LOXL4蛋白的翻译水平。结果 LOXL4 mRNA在三株人宫颈癌细胞中存在不同程度的表达,蛋白翻译水平与mRNA基因表达一致,以Hela表达为最高,ME180次之,HCC94表达最低;而ME180和HCC94中MatriptasemRNA相对表达丰度分别是Hela的4.09倍和12.40倍。结论本研究成功建立了LOXL4及Matriptase在体外培养宫颈癌细胞中表达的检测平台,LOXL4、Matriptase的相对表达水平可能与宫颈癌细胞的侵袭和迁移能力有关。

Objective To investigate the expression of LOXL4 and Matriptase in three human cervical carcinoma cell lines.Methods LOXL4 and Matriptase mRNA levels were tested by quantitave real-time PCR and LOXL4 protein level was investigated by western-blot in cervical carcinoma cell lines Hela, ME180 and HCC94, respectively. Results Differential expression were found both in LOXL4 gene and protein levels of all the three human cervical carcinoma cell linesin vitro. Hela was the highest expression,followed by ME180 and HCC94 was the lowest.The relative abundances of Matriptase in ME180 and HCC94 were 4.09 and 12.40 times as high as that in Hela. Conclusion This study has successfully established detection platformfor LOXL4 and Matriptase gene expression in cervical carcinoma cells. The expressionsof LOXL4 and Matriptase might be associated with invasion and metastasis of cervical cancer cells.