可注射自体脂肪颗粒复合PRF和SVF促进移植脂肪组织再生

The adjuvant use of stromal vascular fraction and platelet-rich fibrin for autologous adipose tissue transplantation

目的：探讨PRF和SVF对自体脂肪颗粒移植的作用，并分析这种可注射复合生物材料的移植效果．方法：健康新西兰家兔24只，分为四组：单纯脂肪组织移植组（2mLAG＋0．2mLNS）、脂肪颗粒复合富血小板纤维蛋白（2mLAG＋0．2mLPRF）组、脂肪颗粒复合基质血管成分（2mLAG＋0．2mLSVF）组和脂肪颗粒复合PRF和SVF[2mLAG＋0．2mL（SVF＋PRF）]组．术后1、3、6月取材，进行大体观察、组织病理学检测、游标卡尺和B超对移植脂肪组织的体积测量．采用SPSS16．0软件，对四组移植脂肪组织的存活率及吸收率进行方差分析并采用LSD法对不同时间点组间进行比较．结果：24周后各组植入脂肪组织的吸收率分别为AG＋NS[（49．4±9．5）％]，AG＋SVF[（27．2±4．4）％]，AG＋PRF[（36．4±8．5）％]和AG＋SVF＋PRF[（17．4±6．2）％]，并有显著差异（P〈0．01）．结论：实验结果表明自体脂肪组织复合PRF和SVF能够促进移植脂肪组织的再生，为临床脂肪组织移植提供一种新的策略．

AIM： This study was to explore the role of PRF and SVF on autologous adipose granule transplant, and analyze the effect of the transplantation of the injectable compound biological material. METHODS： 24 healthy New Zealand rabbits were di vided into four groups ： 2 ml adipose granules and 0.2 ml normal saline solution （ AG ＋ NS group）, 2 ml adipose granules and 0.2 ml SVF （ AG ＋ SVF group）, 2 ml adipose granules and 0.2 ml PRF （AG ＋ PRF group） or 2 ml adipose granules combined with 0.1 ml SVF and 0.1 ml PRF （ AG ＋ SVF ＋ PRF group）. 1, 3, 6 months after the operation, each group was taken gross observa tion, histological examination, and was measured the volume of transplanted adipose tissue by Vernier caliper and B ultrasound. All data collected were expressed as means standard deviation （SD）. Statistical analysis was performed by One-Way ANOVA and LSD method using SPSS 16.0 software. A p-value less than 0.05 was considered statistically significant. RESULTS ： Twenty four weeks post implantation, the resorption rates of implanted tis sue in each group were （49.4 ±9.5% ）, （27.2 ±4.4% ）, （36. 4± 8.5% ） and （ 17.4 ± 6.2% ）, respectively, and were signifi cantly different （P 〈0.01 ）. CONCLUSION： The results dem onstrated that the efficacy of adipose tissue implantation can be enhanced by using autologous PRF and SVF cells as therapeutic adjuvants, offering a clinically translatable strategy for augmenting soft tissue reconstruction.