2型糖尿病患者外周血中hsa—miR-29b的表达及临床意义

Expression and Clinical Significance of Peripheral Has-miR-29b During the Type 2 Diabetes

目的探讨2型糖尿病患者外周血中微小RNA（hsa-miR-29b）的表达及临床意义。方法于201l～2013年期间收集赣州人民医院门诊及住院2型糖尿病患者初诊50例，其中男30例，女20例，年龄35～70岁。并收集同期健康体检者50例作为对照组，其中男25名，女25名，年龄25～75岁。所有患者做糖耐量试验并证实，并运用实时荧光定量RT—PCR检测外周血清中hsa－miR-29b的表达水平，运用两两比较t检验比较糖尿病患者组与健康对照组外周血清中hsa—miR-29b的表达水平。结果健康组hsa—mir-29b的相对表达水平为（13．56±2．11）％，2型糖尿病患者组hsa—mir-29b表达水平为（32．43±3．60）％。2型糖尿病患者外周血清中hsa—miR-29b表达高于正常对照组，差异具有统计学意义（t-20．5，P=0．02）。结论2型糖尿病患者外周血清中hsa—miR-29b水平呈高表达。

Objective To study expression and clinical significance of peripheral diabetes 2 （T2DM）related miR-29b. Methods 50 subjects were recruited,including 30 cases of male,20 cases of female,the age was from 35 to 70. At the same time,col- lecting the healthy physical examination as the control group. Diagnoses were confirmed by a oral glucose tolerance test （OGTT）. Serum miR-29b levels were determined by realtime RT-PCR. Results Serum miR-29b levels showed a increase from T2DM patient. Its expression in T2DM was significantly higher than that health people,while had significant difference （P〈0.05）. Conclusion Serum miR-29b level was increased in type 2 diabetic patient.