快速检测方法筛查粪便中产ESBLs革兰阴性菌调查

Rapid Detection Method of Screening Fecal Middle ESBLs Gram-negative Bacteria Survey

目的了解住院患者粪便中产ESBLs革兰阴性菌比例，为临床制定预防控制措施提供依据。方法采用法国梅里埃公司提供的产色平皿（ESBLs）分离鉴定产ESBLs革兰阴性菌；将2013年3月1～5日200例住院患者入院时采集的粪便常规标本接种在产色平皿上，经35℃，18～24h培养判定结果。结果采集住院患者粪便标本200份，123份标本筛查出产ESBLs革兰阴性菌，住院患者粪便中产ESBLS革兰阴性菌比例为61．50％；其中大肠埃希菌116份、肺炎克雷伯菌6份、大肠埃希菌＋肺炎克雷伯菌1份；儿童阳性率略高于成人（χ^2=0．1082，P〉0．05），男性阳性率略高于女性（χ^2=0．0865，P〉0．05），差异均无统计学显著性意义。结论住院患者粪便中产ESBLs革兰阴性菌比例高，主要为产ESBLs大肠埃希菌，阳性率与年龄、性别关系不大。

Objective To investigate the inpatient fecal middle ESBLs proportion of gram-negative bacilli, provide evidences for the prevention and control measures for clinical. Methods The chromogenic agar France bioMerieux company provided （ESBLs） isolation and identification of ESBLs producing gram negative bacteria. The 2013 March 1-5 of 200 cases were collected in admission of patients withstool specimens were inoculated in the chromogenic agar, after 35 ℃, 18 - 24 hours of training results. Results The acquisition of hospitalized patients stool specimens of 200 copies, 123 specimens of screening ESBLs producing gram negative bacteria, fecal middle ESBLs hospitalized patients with gram-negative bacteria accounted for 61.50 %. Of which 116 Escherichia coli, Klebsiella pneumoniae 6 copies, Escherichia coli ＋ Klebsiella pneumonias 1 ; the positive rate was slightly higher than that of adult children （X2 =0. 108 2,P〉 0.05）,the positive rate of male was higher than that of the female （X2 = 0. 086 5, P〉0.05） and there were no statistical significance. Conclusion Patients with fecal middle ESBLs Gram-negative bacteria ratio was high, mainly for the production of ESBLs in Escherichia coli, the positive rate and age, gender not.