摘要
生物样品图像边缘增强与提取是医学图像处理的关键技术之一,是进行生物样品形态分析的基础。图像边缘增强通常是通过计算机编程对原始图像进行后期处理来实现的,然而,这里应用共焦空间微分显微镜系统,实现了在获得样品共焦显微图像的同时直接获取对应的边缘增强显微图像,且图像分辨率与对比度较高。实验不仅获取了掩膜板与标准分辨率板RTA-07的边缘增强图像,说明系统分辨率达到1.5μm,而且实现了生物细胞的边缘增强图像的获取,如血红细胞与口腔上皮细胞,图像可用于样品尺度与面积等形态的分析与计算,在生物医学研究中有实际应用意义。
Edge enhancement and extraction of biological samples＇ images are one of the key technologies for the processing of medical images, and the basis of samples＇ morphological analysis. In general, the realization for image edge enhancement is through post processing of original images by computer programming. However, spatial differential confocal microscopy system has been applied to directly obtain the high resolution and contrast edge enhanced microscopic images of samples while the confocal microscopic images are obtained. The biomedical application is demonstrated not only for edge enhancement imaging for a mask and a resolution test target （RTA-07） that shows the resolution of the system is 1.5 /~m, but also for edge enhancement imaging for biological cells like RBCs and oral epithelial cells. The obtained edge enhanced microscopic images can be used for simply sample morphological analysis and calculation, such as the scale and area, which has a practical application significance in the research of biomedicine.
出处
《光学学报》
EI
CAS
CSCD
北大核心
2014年第3期209-213,共5页
Acta Optica Sinica
基金
国家自然科学基金(61178086)
广东省自然科学基金重点项目(S2013020012810)
关键词
图像处理
共焦显微镜
边缘增强图像
空间微分技术
细胞
形态分析
image processing
confocal microscopy
edge enhanced images
spatial differential technique
cells
morphological analysis
