摘要
根据黄瓜棒孢叶斑病菌多主棒孢(Corynespora cassiicola)与棒孢属下女贞棒孢(Corynespora lieustri)、威尔士棒孢(Corynespora cambrensis)和其他常见病原真菌Actin基因序列差异,设计多主棒孢的特异性引物Caa5F/Caa5R,建立了黄瓜棒孢叶斑病菌的PCR检测方法,可对引起黄瓜棒孢叶斑病的病原菌扩增出160 bp的特异性条带,检测灵敏性为4 pg·μL-1 DNA,并可从接种后发病的黄瓜叶片总DNA中检测到特异条带。该引物的PCR检测方法灵敏性较高,可直接检测植株总DNA,无需病原菌的分离培养,适用于对黄瓜棒孢叶斑病特异快速的检测。
A rapid PCR detection assay of Corynespora cassiicola, the causal agent of Corynespora leaf spot of cucumber, was developed based on the .Actin gene of the pathogen. Primer pair CaaSF/CaaSR were designed on the different bases of Actin gene sequences between C. cassiicola and other common plant fungal pathogens, including Corynespora lieustri and Corynespora cambrensis, and only C. cassiicola performed a 160 bp amplication. C. cassiicola could be detected in infected cucumber leaves without isolation or cultivation by the PCR assay. The assay with a high sensitivity of 4 pg·μL^-1 DNA per reaction could be a useful tool of rapid detection of Corvnesoora leaf spot of cucumber.
出处
《园艺学报》
CAS
CSCD
北大核心
2014年第3期585-592,共8页
Acta Horticulturae Sinica
基金
国家现代农业产业技术体系建设专项资金项目(CARS-25)
农业部园艺作物生物学与种质创制重点实验室项目
