改性PAMAM促进脱矿牙本质再矿化的体外研究

In vitro study of the effects of modified PAMAM on remineralization of demineralized dentine

目的:探讨羧基改性的PAMAM对脱矿牙本质再矿化的影响。方法:采用磷酸酸蚀后制备的牙本质脱矿模型,并随机分为A(空白对照组)、B(PAMAM组)、C[PAMAM+Ca(OH)2组]3组,再矿化前A组不做任何预处理,B组用PAMAM预处理,C组用PAMAM+Ca(OH)2液预处理,然后各组均于人工唾液中进行再矿化处理2周;分别采用扫描电镜(SEM)、能谱仪(EDS)评估各组原位再矿化效果。结果:SEM观察显示,与A组、B组相比,C组牙本质小管内均形成较厚的沉积物,并与牙本质小管紧密结合,未见牙本质小管开放;EDS显示,A、B、C各组的Ca/P比分别为1.71±0.024、1.79±0.065和1.96±0.081,组间比较P<0.05。结论:羧基改性的PAMAM协同Ca(OH)2液可以有效促进脱矿牙本质的再矿化。

AIM： To study the effect of the earboxyl - modified PAMAM on remineralization of demineralized dentine. METHODS： An in situ remineralization appoach was applied. 9 dentine discs were etched by 37% phosphoric acid and randomly divided into 3 groups （n = 3）. The sampls in group A （control） were directly immersed into artificial saliva, in group B were pretreated by PAMAM and in group C by PAMAM and Ca（ OH）2. Then all the sampls were immersed into artificial saliva for 2 weeks. The remineralization was evaluated by scanning electron micros- copy （SEM） and energy disperse spectroscopy （EDS）. RESULTS： SEM images showed that the dentinal tubules of the sampls in group C were effectively blocked. EDS showed that Ca/P ratio of the sampls in group A, B and C was 1.71 ± 0.024, 1.79 ± 0.065 and 1.96 ± 0.081 respectively（ between each 2 groups, （P 〈 0.05）. CONCLUTION： Carboxy- modified PAMAM plus Ca（OH） 2 can promote the remineralization of demineralized dentine.