婴儿肝炎综合征肝脏巨细胞病毒低基质磷酸化蛋白的检测意义

The Clinical Value of Hepatic Cytomegalovirus pp65 in Diagnosis of Infantile Hepatitis Syndrome

目的:观察人巨细胞病毒(HCMV)低基质磷酸化蛋白(pp65)在婴儿肝炎综合征肝脏组织中的表达,为诊断婴儿巨细胞病毒性肝炎提供科学依据。方法:选择30例婴儿肝炎综合征患儿,在B超介导下进行肝组织活检,免疫组化法检测肝组织中HCMV pp65抗原,观察婴儿巨细胞病毒性肝炎特异性病理改变,同时用ELISA方法检测血清HCMV IgM水平,计算并比较两指标的阳性率。结果:30例婴儿肝炎综合征患儿肝组织HCMV pp65抗原阳性18例,血清IgM阳性6例,阳性率分别为60%、20%。两者阳性率比较差异有显著性(P<0.01)。结论:通过免疫组织化学检测婴儿肝组织中HCMV pp65抗原表达,可用于诊断婴儿巨细胞病毒性肝炎。

Objective：To observe the expression of low substrate phosphorylation protein （pp65） of human cytomegalovirus（HCMV） in liver tissues of infants with hepatitis syndrome,and so as to provide scientific basis for CMV-hepatitis diagnosis.Methods：Thirty infants with hepatitis syndrome were involved,liver biopsy was carried out under b-ultrasound guidance; HCMV pp65 antigen was detected with immunohistochemistry assay,HCMV-IgM level was detected with ELISA; The sensitivity of the two methods was compared.Results：Positive rates of HCMV pp65 antigen and HCMV-IgM were 60% （18/30） and 20% （6/30）,respectively （P 〈0.01）.Conclusions：Testing liver HCMV pp65 antigen with immunohistochemistry assay can offer reliable evidence for CMV-hepatitis diagnosis,having clinical value.