间隙连接蛋白40和45在慢性肾衰大鼠肾组织中的表达及黄芪多糖与水苏碱配伍的干预作用(英文)

Expression of connexin 40 and connexin 45 in renal tissue of rats with chronic renal failure and effect of treatment with Astragalus polysaccharide and stachydrine combination

目的:探究间隙连接蛋白40和45(Cx40和Cx45)在慢性肾衰大鼠肾组织中的表达、黄芪多糖与水苏碱配伍对这2种间隙连接蛋白表达的影响及对慢性肾衰的治疗效果。方法:Wistar大鼠30只,随机分为4组,用腺嘌呤(200 mg·kg-1·d-1)制造慢性肾衰大鼠模型,对照组6只,慢性肾衰模型组8只,黄芪多糖(200 mg·kg-1·d-1)水苏碱(2.5 mg·kg-1·d-1)配伍低剂量治疗慢性肾衰组8只,黄芪多糖(400 mg·kg-1·d-1)水苏碱(5 mg·kg-1·d-1)配伍高剂量治疗慢性肾衰组8只。7周后用免疫组织化学染色的方法观察各组大鼠肾脏中Cx40和Cx45的表达,检测各组的肾脏功能、电解质水平及肾脏病理学变化。结果:模型组大鼠肾脏中的Cx40和Cx45表达相对对照组明显增加,而低剂量配伍组及高剂量配伍组肾脏的Cx40和Cx45表达均明显低于模型组,配伍还可以减少慢性肾衰大鼠肾重量和肾指数,改善肾脏病理学状况,降低血浆电解质、肌酐及尿素氮。结论:慢性肾衰导致大鼠肾脏Cx40和Cx45表达增加,黄芪多糖与水苏碱配伍则可以减少肾脏Cx40和Cx45表达,配伍在增强慢性肾衰肾脏功能及保护肾脏方面有显著的治疗效果。

AIM： To explore the expression of connexin 40 （Cx40） and connexin 45 （Cx45） in chronic renal failure rats, and to investigate the effect of an Astragalus polysaccharide and stachydrine combination on the expressions of the 2 proteins, and the treatment effect Of the combination on chronic renal failure. METHODS ： Wistar rats were randomly divided into 4 groups： control group （6 rats）, chronic renal failure model group i 8 rats）, low-dose Astragalus polysaccha- ride （200 mg. kg-l . d-1 ） and stachydrine （2.5 mg . kg-l . d-1 ） combination group （8 rats）, and high-dose Astragaius polysaccharide （400 mg. kg-1 . d-l ） and stachydrine （5 mg . kg-1. d-1 ） combination group （8 rats）. The rat model of chronic renal failure was induced by adenine （200 mg. kg-1.d-1） garage, Mter 7-week administration, the expression of Cx40 and Cx45 in the renal tissue was determined by immunohistochemical staining. The renal function, electrolyte levels, and pathological changes of the kidneys were also analyzed. RESULTS ： The expression of Cx40 and Cx45 was increased in the renal tissue of chronic renal failure rats. The combination of Astragalus polysaccharide and stachydrine reduced the kid ney weight and index, attenuated the pathological renal damage in the model rats, decreased the expression of Cx40 and Cx45, maintained the stability of serum electrolytes, and decreased the levels of serum creatinine and blood urea nitrogen. CONCLUSION： Chronic renal failure caused the increase in the expression of Cx40 and Cx45 in the rat renal tissue. The combination of Astragalus polysaccharide and stachydrine reduces the expression of Cx40 and Cx45 in the renal tissue, and improves the renal function, thus playing a role in protecting the kidneys.