DRM1,DRM2参与拟南芥愈伤组织的形成

DRM1,DRM2 are involved in the callus formation in Arabidopsis

通过体视显微镜、半薄切片、扫描电子显微镜观察拟南芥DNA从头甲基化酶DRM1,DRM2的双突变体drm1/drm2在愈伤诱导培养基上所诱导的愈伤组织,结果发现:与野生型相比,其种子和根、叶、叶柄等外植体,均具有在同等条件下愈伤组织增多,分裂旺盛的表型。编码拟南芥DNA从头甲基化酶DRM1,DRM2的基因DRM1,DRM2的表达水平也与生长素、细胞分裂素等外源激素浓度相关,由此推测外源激素浓度影响DRM1,DRM2的表达。暗示了DRM1,DRM2可能参与了拟南芥愈伤组织形成过程。

By stereo microscope, scanning electron microscope and transmission electron microscope, the microstructure and ultra structure of cells from callus can be observed. Results show that the seedlings of Arabidopsis mutant drm1/drm2, lacking the de novo DNA methyltransferase DRM1, DRM2, can be induced to form more callus compared to the wild type （ WS） on Callus-inducing-Medium （CIM, containing 1 mg/L 2,4-D, 0.1 mg/L KT ）. Other different explants of roots, leaves and petioles were used to compare with the seedlings and obtained the same results. The molecular results show the expression levels of genes DRM1,DRM2 coding the de novo DNA methyltransferase DRM1,DRM2 are related to the concentration of hormones （auxin and cytokinin） which can induce the formation of callus. So it can be indicated that DRM1,DRM2 are possibly involved in the callus formation.