丙型肝炎病毒核心蛋白对人源性肝细胞凋亡的影响

Effect of hepatitis C virus core protein on the apoptosis of human hepatocytes

目的研究丙型肝炎病毒(HCV)核心蛋白对人源性肝细胞凋亡的影响。方法构建HCV核心蛋白真核表达载体pIRES-core,转染人源永生化肝细胞QSG7701,Western blotting印记法检测核心蛋白及转录因子NF-κB的表达;流式细胞仪检测细胞凋亡率。结果 QSG7701/core组细胞凋亡率为(1.34±0.07)%,低于QSG7701组(2.35±0.11)%和QSG7701/pcDNA3.1组(2.58±0.13)%,三组比较差异有统计学意义(P<0.05);同时转录因子NF-κB表达上调。结论 HCV核心蛋白上调转录因子NF-κB的表达,可能是抑制人源性肝细胞QSG7701凋亡的主要机制。

Objective To investigate the effects of hepatitis C virus （HCV） core protein on the apoptosis of human hepatocytes. Methods The eukaryotic expressing vector for HCV core protein was constructed, and transfected with QSG7701 cells. The expressions of core protein and NF-KB were detected by Western blotting. The apoptosis rate of QSG7701 cells was measured with flow cytometry. Results The apoptosis of QSG7701/core was （ 1.34 ± 0.07 ） %, lower than （2.35 ± 0.11 ） % of QSG7701 and （ 2.58 ± 0.13 ） % of QSG7701/pcDNA-3.1, with statistical significant difference （P 〈 0.05）. Besides, the transcription factor NF-KB was activated. Conclusion HCV core protein suppresses apoptosis of QSG7701 possibly by activating the transcription factor NF-kB.