正交优化白及多糖复合支架材料的实验研究

Experimental study of the bletilla striata glucomanan composited scaffolds by orthogonal experiment

目的研究制备用于骨组织工程再生的白及多糖复合支架材料,采用正交实验的方法优化最佳配比。方法将纯化的白及多糖用高碘酸钠氧化改性,并与羟丙基壳聚糖和纳米珍珠层粉共混,冷冻干燥法得到复合支架材料,测试材料性能。结果研究制备出白及多糖复合支架材料并得出最优比例,支架材料各项指标符合组织工程要求。结论成功用正交分析方法优化了白及多糖复合支架材料,为其进一步研究提供了理论依据,有望成为新型组织工程生物支架材料。

Objective To study the preparation of bletilla striata glucomanan composited scaffolds used for bone tissue regeneration and to analyze its optimum proportion by orthogonal experiment. Methods Bletilla striata glucomannan was oxidized by sodium periodate, mixed with hydroxypropyl chitosan and nano-nacre powder, and then the scaffold was created by freeze-dry method, and its properties were tested. Results Bletilla striata glucomanan composited scaf- folds was synthesized and optimized. All its properties met the requirements of tissue engineering. Conclusion This study optimized Bletilla striata glucomanan composited scaffolds successfully by orthogonal experiment. It provided a theoretical basis for further research on the scaffold, which may become a new material for bone tissue regeneration.