水稻病程相关PR1家族蛋白质在叶片生长及与白叶枯病菌互作反应中的表达

Expression Analysis of Pathogenesis-related 1 Proteins in Normal Growth of Rice Leaves and Interactions Between Rice and Xanthomonas oryzae pv. oryzae

病程相关(PR)蛋白质经常被用作抗病反应的分子标记。利用免疫印迹(WB)技术检测了7个PR1家族蛋白质在水稻(Oryza sativa)叶片生长及与白叶枯病菌互作反应过程中的表达,发现6个PR1家族蛋白质在叶片生长中有表达。检测PR1蛋白质在Xa21介导的抗白叶枯病过程中的表达,结果显示PR1#052、PR1#072、PR1#073和PR1#121四个蛋白质在抗病反应后期呈上调或诱导表达,PR1#071则表达下调。进一步比较它们在抗病、感病和对照(Mock)反应中的表达丰度,发现在抗病和感病反应中的变化幅度均明显大于对照反应,推测这些PR蛋白质在水稻-白叶枯病菌互作反应中发挥作用。另外,对PR1基因上游启动子区的cis元件进行了分析。该研究初步揭示了水稻PR1家族蛋白质的表达谱,为进一步了解PR1蛋白质的功能提供了线索。

Pathogenesis-related （PR） proteins are commonly used as markers of plant defense responses. To examine the expression patterns of rice PR proteins, we used western blot analysis to investigate rice PR1 family proteins and found 6 of 7 tested PR1 proteins expressed in rice leaves under normal growth. We examined the expression pattern of PR1 proteins in the interaction between rice and the bacterium Xanthomonas oryzae pv. oryzae （Xoo） and found PR1#052, PR1#072, PR1#073 and PR1#121 upregulated or induced in the later stage of Xa21-mediated incompatible interactions and PR1#071 downregulated. The comparison among resistance, susceptible and mock treatments demon- strated that the alteration in abundance was greater with resistance and susceptible than mock treatment, so PR1 pro- teins may play a role in rice under biotic stress. In addition, we predicted cis elements in the upstream promoter region of PR1 genes. We reveal the expression spectrum of rice PR1 family proteins and provide useful clues for the functional investigation of rice PR1 proteins.