活体成像技术监测肿瘤生长动态的研究

Study on the monitor effect of in vivo imaging technique on tumor growth dynamic

目的生长动态的监测是肿瘤学重要的研究内容之一,现有的各种生长动态的监测方法存在诸多缺陷。文中研究活体成像技术(in vivo imaging technique,IVIT)在肿瘤生长动态监测中的应用。方法在18只Babl/C小鼠背部皮下注射1×106/mL B16-F10-luc-G5细胞100μL,建立种植瘤模型。肿块出现后每天测量肿瘤并计算肿瘤体积,并于第0、3、5、7、9、14天每次取3只小鼠以活体成像系统(in vivo imaging system,IVIS)检测肿瘤生物发光,检测后处死取肿瘤组织制成石蜡切片行病理学检查。结果所有小鼠接种成功肿瘤,第5天肿瘤可见。第5、7、9、14天肿瘤大小分别为[(2.86E+00)±1.21]、[(4.87E+00)±1.66]、[(9.27E+01)±6.31]、[(2.60E+02)±7.88]mm3;所有移植瘤生物发光均被IVIS检测到,第0、3、5、7、9、14天肿瘤平均实测光子数为[(6.35E+05)±7655]、[(1.12E+05)±1820]、[(1.62E+05)±2090]、[(2.40E+05)±3515]、[(1.18E+06)±11530]、[(2.23E+06)±17934]photon/(cm2·ser·s);移植瘤平均实测光子数与体积之间存在线性回归关系(R2=0.97);以IVIS监测结果绘制的肿瘤生长曲线,揭示了B16F10移植瘤生长动态特点。结论 IVIT可连续监测标记肿瘤的生长动态,准确灵敏、具有一定实用价值。

Objective The inspection of growth dynamic is an important part in onocology. The aim of this study is to ex-plore the effect of in vivo imaging technique （IVIT） on tumor growth dynamic monitoring. Methods Subcutaneous injection of B16-F10-1uc-G5 tumor cells （ 1 × 10^5 in 100 μL） in eighteen Babl/C mice to establish implantation tumor model. The tumor volume was calculated daily after the production of the inoculated tumor. Tumor bioluminescence of three mice were detected by in vivo imaging sys-tem （IVIS） on days 0, 3, 5, 7, 9 and 14, and paraffin section of tumor tissues was used to complete pathological examination. Results Tumors were successfully inoculated in the mice, which emerged on day 5. The inoculated tumor-volume at days 5, 7, 9, 14 were （2.86E ＋00） ±1.21 mm^3, （4.87E ＋00） ±1.66 mm^3, （9.27E ＋01） ±6.31 mm^3 and （2.60E ＋02） ±7.88mm^3, respective-ly. All the inoculated tumor bioluminescence wase detected by IVIS. The luminescence of tumor-volume on days 0, 3, 5, 7, 9, 14 was （6.35E ＋05） ±7655, （1.12E ＋05） ± 1820, （1.62E ＋05） ±2090, （2.40E ＋05） ±3515, （1.18E ＋06） ± 11 530 and （ 2.23 E ＋ 06） ± 17 934 photon/（ cm^2 · ser · s）. A significant correlation between tumor-volume and bioluminescence （ R2 = 0.97 ） was demonstrated. Tumor growth curve according to the results of IVIT can reveal the growth characteristics of B16F10 inoculated tumor. Conclusion IVIT is an effective method for monitoring tumor growth dynamic with high specificity and sensitivity.