小叶兜兰的种子发育和无菌萌发

Seed Development and Asymbiotic Germination of Paphiopedilum barbigerum Tang et Wang

为建立小叶兜兰的繁育技术体系,本研究通过无菌播种的方法,辅以TTC生活力测定等方法,比较了小叶兜兰种子在授粉后不同发育时期和培养条件下的萌发率,对小叶兜兰种胚的发育过程进行了显微观察,探讨种胚发育程度与萌发的关系。结果表明,小叶兜兰种胚的发育阶段对萌发的影响最大,授粉后255 d的种子萌发率最高(90.71%),该阶段种子仍呈白色但微干燥,种胚刚发育至球形胚阶段,胚柄尚存。1/4MS和1/2MS为小叶兜兰适宜的基本培养基,添加100 mg·L-1的土豆汁对小叶兜兰的无菌萌发有良好的促进作用。

In order to establish the propagation techniques of Paphiopedilum barbigerum, we compared the germination rates of seeds after pollination in different development stages and cultures, observed the process of embryo development by microscope with the method of asymbiotic seed germination and TTC test. The relationship between asymbiotic germination rate and the anatomical features in seed development of P. barbigerum was also studied. The results indicated that the development stage was the most important factor in germination. And the highest rate of seed germination was obtained in 255 days after pollination （DAP） with the white and little dehydrated seeds. Moreover, the embryo began to take an ellipsoidal shape, and the suspensor cells were still present. 1/4MS and 1/2MS medium were both suitable for germination, and the addition of 100 mg·L^-1 potato juice had a good effect on germination.