外源突变基因AP1M2转化毛白杨的研究

Genetic Transformation of Populus tomentosa Carr. with the Foreign Mutant Gene AP1M2

为探究毛白杨开花调控分子机制并获得不飞絮毛白杨株系,利用农杆菌介导法将显性负突变结构基因AP1M2转入毛白杨中,经PCR检测,共获得阳性转化植株7株。通过RT-qPCR检测转基因植株中外源基因AP1M2表达量,发现各株系间的表达水平差异显著,最高为内参的5.41倍,最低为1.89倍。对转基因毛白杨中内源PtAP1和其他开花关键基因的表达量进行检测,发现内源AP1的表达受到抑制,表达量明显下调;其他开花关键基因LFY、AP3、PI、SEP3和FT1等的表达量也有不同程度的降低,而FT2表达量并没有明显变化。这些研究结果表明转AP1M2毛白杨中AP1、LFY、AP3、PI、SEP3和FT1的表达受到明显抑制,对毛白杨花发育将有一定影响。

In order to study the mechanism of flowering in Populus tomentosa and obtain sterile poplar, the foreign mutant gene AP1M2 was transformed into P. tomentosa via the Agrobacteriurn-mediated method. The positive results verified by PCR showed that seven transgenics lines obtained. RT-qPCR results showed that the expression levels in seven transgenic lines were different. The highest level of expression was 5.41 times and the lowest level was 1.89 times compared to the reference gene. The expression ofAP1, LFY, AP3, PI, SEP3 and FT1 in transgenic poplars was down-regulated obviously, but the FT2 expression level was not changed. These results suggested that the AP1, LFY, AP3, PI, SEP3 and FT1 were suppressed.