促性腺激素释放激素受体在雌性山羊颈胸神经节中的分布

Distribution of gonadotropin releasing hormone receptor in cervicothoracic ganglion of female goats

目的探讨颈胸神经节是否具备接受促性腺激素释放激素(GnRH)作用的条件。方法实验采用免疫组织化学SP法,观察GnRH受体在雌性山羊颈胸神经节中的分布特点。结果在颈胸神经节中,神经元胞体均为GnRH受体免疫反应阳性,其细胞膜以及膜下胞质中的颗粒状物质呈棕黄色,GnRH受体为强阳性表达,而胞核呈阴性表达。部分神经元可见其突起,呈黄色,GnRH受体为中等阳性表达。卫星细胞也呈黄色,GnRH受体为中等阳性表达。图像分析表明,呈阳性反应的神经元胞体与节内其他阳性成分相比,其GnRH受体相对表达量差异性极显著(P<0.01)。结论在雌性山羊颈胸神经节中,神经元胞体是GnRH发挥作用的主要靶细胞,颈胸神经节具备接受GnRH作用的条件。提示GnRH有可能通过作用于颈胸神经节交感节后神经元上的GnRH受体来影响心肺器官的功能活动,即颈胸神经节可能是心肺功能活动中协调GnRH内分泌调节和该神经节自主神经调节两途径之间的中继站。

Objective To explore whether cervicothoracic ganglion has the condition by which gonadotropin releasing hormon（GnRH） can exert its function. Methods Immunohistochemical SP method was adopted to observe the distribution of GnRH receptor （GnRHR） in cervicothoracic ganglion of female goats. Results The results showed that, all the neurons in eervicothoracic ganglion responded positively to GnRHR. The membrane of neurons and the granular material in cytoplasm were strong positive. The neuronal nucleus was negative to GnRHR while neurites and satellite cells showed medium positive. Image analysis showed a significant difference of GnRHR expression between neurons and non-neurons （P 〈 0.01 ）. Conclusion The results indicate that neurons in cervicothoracic ganglion are the main target cells of GnRH, and cervicothoracic ganglion has the condition by which GnRH can exert its function, which implies GnRH may regulate the function of heart and lung through the GnRHR distributed in eervieothoracie ganglion. Thus,the GnRHR existing in these neurons may be a ＂transfer station＂ for GnRH to coordinate heart and lung activity through endocrine and autonomic nerve pattern.