巢蛋白及性别决定基因高迁移率组蛋白在SAMP8小鼠穹隆下器的表达及意义

Expression and significance of Nestin and SOX2 in the SAMP8 mice subfornical organ

目的观察巢蛋白(Nestin)与性别决定基因高迁移率组蛋白(SOX2)在快速老化小鼠SAMP8穹隆下器(SFO)中的表达。方法成年8月龄SAMP8小鼠设为实验组,采用免疫组织化学和免疫组织化学荧光染色的方法观察Nestin及SOX2在穹隆下器中的表达,同时设正常老化小鼠SAMR1对照。结果 Nestin免疫组织化学染色发现,对照组免疫阳性细胞呈突起分布,放射丝状表达;实验组免疫阳性细胞染色深,丝状结构粗大,在血管周围密集分布,阳性细胞百分比(49.17±7.60)%较对照组阳性细胞百分比(16.33±4.41)%明显增多,差异有统计学意义(P<0.01)。SOX2免疫组织化学染色,对照组免疫阳性细胞散在分布,染色深浅不一;实验组大部分阳性细胞染色较深,在血管周围密集分布;阳性表达细胞百分比(62.17±20.27)%较对照组阳性表达细胞百分比(36.00±16.20)%明显增多,差异有统计学意义(P<0.05)。荧光双标染色,对照组SOX2散在分布,其间可见Nestin阳性表达,室管膜下区可见少量SOX2/Nestin双表达细胞。实验组阳性表达强烈,SOX2/Nestin双表达细胞增多。结论 Nestin及SOX2在快速老化小鼠SAMP8穹隆下器的表达明显增强,表明阿尔茨海默病(AD)在一定时期可能引起穹隆下器神经干细胞/祖细胞的增殖或分化增强,进而可能影响穹隆下器的神经生发功能。

Objective To observe the expression of nestin and sex-determining genes in high-mobility group proteins in the senescence accelerated mouse prone 8 subfornical organ. Methods Adult SAMP8 mice （8 months old） were used. The expressions of Nestin, SOX2 in subfornical organ were observed by using immunohistochemical staining and immunofluorescence staining methods. Normal senescence mouse resistance 1 was displayed as control. Results Nestin immunohistochemical staining of the control group： positive cells were protuberantly distributed and expressed filiform texture with radiate. Nestin immunohistochemical staining of the experimental group： positive cells body stained stronger, filiform texture was thick, densely distributed in surrounded by vessels. The percentage of positive cells（49. 17 ± 7.60）% increased significantly than control group （ 16. 33 ± 4.41 ） % and had statistical significance （ P 〈 0.01 ）. SOX2 immunohistoehemical staining of control group： positive cells stained irregularity and dispersedly distributed. SOX2 immunohistochemical staining of the experimental group： most of positive cells stained deeper, densely distributed in surrounded by vessels. The percentage of positive cells （62.17 ± 20.27 ） % increased significantly than control group （36.00 ± 16.20）% and had statistical significance （P 〈 0.05 ）. Fluorescence double staining in the control group： SOX2 stained irregularity and Nestin expressed during it. A small amount of SOX2 and Nestin double-positive cells were in the subependymal region. In the experimental group： positive cells expressed intensity and showed more double-positive cells. Conclusion Expression of nestin and SOX2 enhances significantly in the senescence accelerated mouse prone 8 subfornical organ, which indicates AD may result in increased number of neural stem/progenitor cell proliferation or differentiation in subfornical organ at same stage, and then the neurogenesis of subfornical organ may be affected in Alzheimer disease.