小鼠海马发育中Bcl-2和Bax表达的体视学观察

Stereoscopic study on the expression of Bcl-2 and Bax in development of mice hippocampus

目的探讨B淋巴细胞瘤-2基因(Bcl-2)和促凋亡基因Bax(Bax)在C57/BL6小鼠海马发育过程中的表达变化。方法取胚龄(E)18、20 d和生后(P)1、3、7、14、21、28 d以及2、3、6、15、18个月的C57/BL6小鼠海马,每组8只,应用免疫组织化学技术及体视学方法检测Bcl-2和Bax蛋白的表达。结果 E18 d^P21 d,在齿状回(DG)的颗粒层、海马阿蒙角(CA)以及CA各区(CA1~CA4)的锥体层,Bcl-2和Bax阳性细胞及其体密度均呈现出先逐渐增加再逐渐降低的趋势,除DG区Bax阳性细胞及其体密度在P14 d达到最高外,其他均在P7 d达到最高(P<0.01)。P28 d后均趋于稳定(P>0.05)。CA锥体层及DG颗粒层Bcl-2/Bax的体密度比值在P1d显著降低(P<0.01),之后趋于稳定(P>0.05)。结论小鼠海马Bcl-2和Bax的表达在胚胎发育晚期和生后发育早期较多,而在成年及老年期的表达稳定在较低水平,它们可能参与了海马的塑形过程。

Objective To observe the expression of Bcl-2 and Bax in the development of C57/BL6 mice hippocampus. Methods Mice hippocampus of Embryos （E） 18 day （d）, E20 d, postnatal （P） 1 d, P3 d, P7 d, P 14 d, P28 d, P2 month （M）, P 3 M, P 6 M, P 15 M, P 18 M were used in this study. Immunohistochemistry and stereoscopy were used to detect the expression of Bcl-2 and Bax protein in the mice hippocampus at different ages. Results From El8 d to P21 d, Bcl-2 and Bax immunoreactive cells in both Ammon＇ cornu （CA） and dentate gyrus （DG） firstly and gradually increased and then decreased（P 〈 0.01 ）. The volume density of both Bcl-2 and Bax immunoreactive cells came to the top on P7 d except the Bax expression in DG which was the highest on P14 d（ P 〈 0.01 ）. Conclusion The expression of Bcl-2 and Bax is high at the early stage of hippocampus development and then low at its adult stage and senectitude, which may be involved with the forming of hippocampus.