牙龈卟啉单胞菌感染对大鼠血管平滑肌细胞细胞间黏附分子-1表达的影响

Effects of Porphyromonas gingivalis infection on intercellular adhesion molecule-1 expression in rat vascular smooth muscle cells

目的观察牙龈卟啉单胞菌ATCC 33277感染对大鼠血管平滑肌细胞(VSMC)细胞间黏附分子-1(ICAM-1)表达的影响。方法建立体外牙龈卟啉单胞菌感染大鼠VSMC模型,逆转录-聚合酶链反应(RT-PCR)检测ICAM-1基因的表达。结果牙龈卟啉单胞菌感染VSMC 8、16、24 h后,ICAM-1表达明显增多,与空白对照组比较,差异有统计学意义(P<0.05)。感染16 h达高峰,感染8 h与感染16、24 h比较,差异有统计学意义(P<0.05)。结论牙龈卟啉单胞菌感染可引起VSMC ICAM-1高表达,这提示牙周致病菌可能参与血管壁的炎症反应,在动脉粥样硬化的发生、发展中有重要的意义。

Objective To observe the effects ofPorphyromonas gingivalis （P. gingivalis）ATCC 33277 infection on ex- pression of intercellular adhesion molecule-1 （ICAM-1） in rat vascular smooth muscle cells（VSMC）. Methods An infection model of rat VSMC invaded by P. gingivalis was established in vitro. The mRNA of ICAM-1 was measured through reverse transcription-polymerase chain reaction （RT-PCR）. Results Compared with the control group, an apparent and statistically significant increase in expression of ICAM-1 mRNA was observed after 8, 16, and 24 h in P. gingivals-infected rat VSMC （P〈0.05）. The expression reached its peak at 16 h, Statistically significant differences were observed in the 8 h group and in the other two experimental groups （P〈0.05）. Conclusion Infection ofP. gingivals in rat VSMC can cause increased expres- sion of ICAM-1, which may have an important function in the progression of atherosclerosis.