格列美脲对高糖环境下大鼠下颌骨成骨细胞葡萄糖摄取的影响

Effect of glimepiride on the glucose uptake of rat mandibular osteoblasts in hyperglycemia

目的格列美脲对高糖环境下大鼠下颌骨成骨细胞葡萄糖摄取的影响。方法分离培养大鼠下颌骨成骨细胞,分别用不同糖浓度(5.5、16.5 mmol·L-1)处理细胞,持续24 h,加入或不加入10μmol·L-1格列美脲干预120 min。采用氟-18标记的脱氧葡萄糖(18F-FDG)测定成骨细胞的葡萄糖摄取,Western blot检测葡萄糖转运蛋白(GLUT)-1、GLUT-3表达水平。结果高糖浓度降低了成骨细胞的糖摄取能力和GLUT-3的表达,增加了GLUT-1的表达。格列美脲能够提高2种糖浓度下成骨细胞糖摄取的能力和GLUT-1、3的表达。结论格列美脲能够促进2种糖浓度下大鼠下颌骨成骨细胞的葡萄糖转运。

Objective To explore the effect of glimepiride on the glucose uptake as well as glucose transporter （GLUT）-1 and GLUT-3 expression levels of rat mandibular osteoblasts in hyperglycemia. Methods Primary osteoblasts were isolated and cultured. Then, the cells were placed in an osteogenic medium containing two glucose concentrations （5.5 and 16.5 mmol·L^-1）, with or without glimepiride （10 μmol·L^-1）. Glucose uptake was determined by employing ^18F-deoxyglucose （^18F-FDG） in the cells, and GLUT-1 and GLUT-3 expression levels were evaluated by Westem blot analysis. Results Glucose at 16.5 mmol·L^-1 significantly inhibited ^18F-FDG uptake and downregnlated GLUT-3 protein expression in osteoblasts. Hyperglycemia in- creased GLUT-1 protein expression. Glimepiride significantly increased glucose uptake and upregnlated GLUT-1 and GLUT-3. Conclusion Glimepiride enhance the glucose transporter in rat osteoblasts at two different glucose concentrations.