新疆地区不同民族GGCX rs 11676382基因多态性分析

Analysis of Gene Polymorphism of GGCX rs11676382 in Different Na-tionalities in Xinjiang

目的了解γ-谷氨酰羧化酶（γ-glutamine carboxylase，GGCX）基因多态位点rs11676382在新疆维吾尔族、汉族人群中的分布情况及其关联性。方法以维吾尔族、汉族获得华法林稳定剂量的患者各100例作为病例组，以维吾尔族、汉族健康人群各100例作为对照组，所有人群均从外周静脉血中提取基因组DNA，采用聚合酶链反应／限制性片段长度多态性（PCRRFLP）技术检测GGCX基因rs11676382位点基因型及等位基因频率，比较维吾尔族、汉族之间基因多态性的差异。结果①获得了新疆地区维吾尔族、汉族人群的GGCX基因rs11676382位点多态性分布；②病例组和对照组GGCX基因rs11676382位点基因型频率和等位基因频率比较有统计学意义（P〈0．05）；维吾尔族病例组和汉族病例组GGCX基因rs11676382位点基因型频率和等位基因频率比较差异有统计学意义（P〈0．05）。结论获得了GGCX基因rs11676382位点在新疆地区不同民族的等位基因和基因型的分布。以及该基因位点在维吾尔族、汉族获得华法林稳定剂量患者中的分布存在民族差异。

Objective To understand the distribution and association of gene polymorphism of gamma-glutamyl carboxylase （GGCX） rs11676382 in Uygur and Han population in Xinjiang. Meth-oils DNA was extracted from the peripheral venous blood of 200 patients who had stable warfa-rin dose （100 Uygurs and 100 Hans） and 200 healthy volunteers （100 Uygurs and 100 Hans） . The allelic and genotypic frequency of GGCX gene 11676382 was detected by PCR restriction fragment length polymorphism （PCRRFLP） . Results 1. The allelic and genotypic frequency distribution of GGCX rs11676382 polymorphism was obtained in Uygurs and Hans in Xinjiang. 2. There was signif-icant difference in allelic and genotypic frequency of GGCX rs11676382 polymorphism between case group and control group （P 〈 0. 05） . In the case group, there was also significant difference be-tween Uygurs and Hans （P 〈 0. 05） . Conclusion The allelic and genotypic frequency distri-bution of GGCX rs11676382 polymorphism in different nationalities in Xinjiang was obtained. There was ethnic difference in GGCX gene 11676382 polymorphic site between Uygur al^d Han patients who had stable warfarin dose.