急性胰腺炎患者血清NF-κB和相关细胞因子表达的变化

Changes of NF-κB and related cytokines expressions in patients with acute pancreatitis

目的探讨血清核转录因子κB(NF-κB)和相关细胞因子在急性胰腺炎(AP)患者中的变化。方法 53例AP患者分为轻症AP(A组,28例)和重症AP(SAP,B组,25例);以20例健康体检者作为对照(C组)。分别于入院第1天、第5天和第10天采用ELISA法检测血清中TNF-κB、IL-6、IL-8、IL-10浓度;提取外周血单个核细胞(PBMC),采用免疫细胞化学定位法检测NF-κB的表达;并进行相关性分析。结果 A、B组血清NF-κB、TNF-α、IL-6、IL-8水平均有先升高后下降的趋势,B组第5天高于A组(P<0.05)。B组IL-10水平较A、C组明显呈上升趋势,第10天达顶峰(P<0.05)。B组血清NF-κB与TNF-α、IL-6和IL-8均呈正相关(P<0.05)。结论 NF-κB可通过转录调控TNF-α、IL-6、IL-8等促炎细胞因子,在AP的病程发展中发挥重要的作用。抗炎细胞因子IL-10可能参与了SAP早期病程的发展,提示了SAP的免疫紊乱。

Objective To study the changes of nuclear factor kappa B（NF-κB） and related cytokines in the patients with acute pancreatitis （AP）. Methods Fifty-three AP cases were divided into two groups of A（with mild AP, 28 cases） and B（with severe AP, 25 cases）. Twenty healthy persons were taken as the controls （group C）. ELISA was performed to detect serum levels of interleukin （IL）-6, IL-8, IL-10 and tumor necrosis factor-α（TNF-α） on the 1st, 5th, and 10th day after admission. The expression of NF-κB in peripheral blood mononuclear cells was measured by immunocytoche-mistry as well. The correlations between NF-rB and related cytokines were analyzed. Results Serum levels of NF-κB,TNF-a,IL-6 and IL-8 in groups of A and B were increased at first, but then decreased,which on the 5th day were higher in group B than those in group A（P〈0. 05）. The IL-10 expression in group B was increased, which peaked on the 10th day and higher han that in groups of A and C（P〈0. 05）. The expression of NF-gB was positively correlated to that of TNF-a, IL-6, and IL-8（P〈0. 05）. Conclusion NF-κB plays an important role in the pathogenesis of AP by modulating the expressions of TNF-α, IL-6, and IL-8. Anti-inflammatory factor IL-10 may participate in the early phase of severe AP, implying the disturbance of immunity.