腺样囊性癌嗜神经侵袭体外模型的建立

Construction of PNI in Vitro Model of Adenoid Cystic Carcinoma

目的:期望建立一种腺样囊性癌嗜神经侵袭的体外研究模型。方法:通过将腺样囊性癌细胞系ACC-M与小鼠背根神经节在基质胶中进行共培养,采用倒置相差显微镜和荧光双染动态观察肿瘤细胞与神经轴突的相互作用过程。结果:通过对腺样囊性癌嗜神经侵袭的体外模型的观察,发现嗜神经侵袭过程的发生是肿瘤细胞与神经之间特异性的相互作用。结论:本研究的腺样囊性癌-背根神经节共培养能够模拟癌细胞嗜神经侵袭的发生过程,对于研究腺样囊性癌嗜神经侵袭的分子机制具有重要价值。

Objective： To construct an in vitro PNI model of ACC, mouse dorsal root ganglia （DRG） and human ACC cell lines （ACC--2 and ACC--M） were co--cultured in Matrigel matrix. Methods： The interaction between cancer cells and nerves were observed through contrast phase microscope and identified by immunofluorescence double staining. The PNI in vitro model could simulate the process of perineural invasion. Results： The in vitro model of ACC showed the tumor cells and nerves were interacted with each other during the perineural invasion process. Conclusion： This study showed the utility of ACC cell--DRG coculture model to study molecular mecha- nism of perineural in ACC.